Meyer-Lueckel H, Tschoppe P, Kielbassa A M
Department of Operative Dentistry and Periodontology, University School of Dental Medicine, Universitätsmedizin Berlin, Berlin, Germany.
J Oral Rehabil. 2006 Oct;33(10):760-6. doi: 10.1111/j.1365-2842.2006.01652.x.
Remineralization might be hampered by various polymers used in saliva substitutes. Thus, the present study evaluated the effects of various calcium and phosphate concentrations of linseed-based solutions on the mineral loss of pre-demineralized bovine enamel in vitro. A commercially available saliva substitute (Salinum) based on linseed was tested as well. Enamel specimens were prepared from bovine incisors and embedded in epoxy resin. One-third of each sample was covered with nail varnish (control of sound enamel). After demineralization (37 degrees Celsius; pH 5.0; 14 days) another third of the samples was nail-varnished again. Subsequently, the specimens (n = 10) were exposed to 12 linseed-based solutions (Ca(2+) addition 0-2 mM; PO4(3-) addition 0-3.2 mM) at pH 5.5 and 6.5 as well as to Salinum) for 14 days (37 degrees Celsius). The differences in mineral loss between the values prior to and after the storage in the various solutions were evaluated from microradiographs of thin sections (100 mum). The general linear model revealed a significant dependency for the mineral loss on 'calcium' (P = 0.003), but not on 'pH' (P = 0.397) and 'phosphate' (P = 0.094). Salinum) induced a significant greater mineral loss compared with equivalently saturated solutions (P < 0.05; anova, Bonferroni). The solution with the highest calcium and phosphate concentration showed the greatest mineral gain (P = 0.033; paired t-test). The addition of calcium and phosphate seems to have a positive effect on the remineralizing qualities of linseed-based saliva substitutes.
唾液替代品中使用的各种聚合物可能会阻碍再矿化。因此，本研究在体外评估了不同钙磷浓度的亚麻籽基溶液对预脱矿牛牙釉质矿物质流失的影响。还测试了一种市售的基于亚麻籽的唾液替代品（Salinum）。从牛切牙制备牙釉质标本并嵌入环氧树脂中。每个样本的三分之一用指甲油覆盖（完好牙釉质的对照）。脱矿后（37摄氏度；pH 5.0；14天），另外三分之一的样本再次用指甲油覆盖。随后，将标本（n = 10）暴露于pH值为5.5和6.5的12种亚麻籽基溶液（添加Ca(2+) 0 - 2 mM；添加PO4(3-) 0 - 3.2 mM）以及Salinum中14天（37摄氏度）。根据薄片（100μm）的显微放射照片评估在各种溶液中储存前后矿物质流失值的差异。一般线性模型显示矿物质流失对“钙”有显著依赖性（P = 0.003），但对“pH”（P = 0.397）和“磷酸盐”（P = 0.094）没有依赖性。与等效饱和溶液相比，Salinum引起的矿物质流失显著更大（P < 0.05；方差分析，Bonferroni检验）。钙和磷酸盐浓度最高的溶液显示出最大的矿物质增加（P = 0.033；配对t检验）。钙和磷酸盐的添加似乎对亚麻籽基唾液替代品的再矿化质量有积极影响。
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