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[甜菊糖苷和莱鲍迪苷A的联合酶法修饰]

[Combined enzymatic modification of stevioside and rebaudioside A].

作者信息

Kochikian V T, Markosian A A, Abelian L A, Balaian A M, Abelian V A

出版信息

Prikl Biokhim Mikrobiol. 2006 Jan-Feb;42(1):37-43.

DOI:
PMID:16521574
Abstract

Cyclodextrin glucanotransferases (CGTases, EC 2.4.1.19) produced by mesophilic, thermophilic, alkaliphilic, and halophilic bacilli were used for transglycosylating stevioside and rebaudiosides A with the use of starch as a donor. CGTases produced by B. stearothermophilus B-5076 B. macerans BIO-4m were the most effective biocatalysts. This method can be successfully used for direct transglycosylation of stevia extract without purification of its individual components.

摘要

嗜温、嗜热、嗜碱和嗜盐芽孢杆菌产生的环糊精葡糖基转移酶（CGTases，EC 2.4.1.19）被用于以淀粉作为供体对甜菊糖苷和莱鲍迪苷A进行转糖基化反应。嗜热脂肪芽孢杆菌B-5076和浸麻芽孢杆菌BIO-4m产生的CGTases是最有效的生物催化剂。该方法可成功用于甜叶菊提取物的直接转糖基化反应，而无需对其单个成分进行纯化。

相似文献

[1]
[Combined enzymatic modification of stevioside and rebaudioside A].

Prikl Biokhim Mikrobiol. 2006

[2]
[Transglycosylation of stevioside by cyclodextrin glucanotransferases of various group of microorganisms].

Prikl Biokhim Mikrobiol. 2004

[3]
Transglycosylation of stevioside to improve the edulcorant quality by lower substitution using cornstarch hydrolyzate and CGTase.

Food Chem. 2012-11-12

[4]
Synthesis of rebaudioside-A by enzymatic transglycosylation of stevioside present in the leaves of Stevia rebaudiana Bertoni.

Food Chem. 2016-1-9

[5]
Downstream processing of stevioside and its potential applications.

Biotechnol Adv. 2011-6-25

[6]
Evaluation of steviol and its glycosides in Stevia rebaudiana leaves and commercial sweetener by ultra-high-performance liquid chromatography-mass spectrometry.

J Chromatogr A. 2010-1-6

[7]
High efficiency transformation of stevioside into a single mono-glycosylated product using a cyclodextrin glucanotransferase from Paenibacillus sp. CGMCC 5316.

World J Microbiol Biotechnol. 2015-12

[8]
Stevioside and related compounds: therapeutic benefits beyond sweetness.

Pharmacol Ther. 2009-6

[9]
Metabolism of stevioside and rebaudioside A from Stevia rebaudiana extracts by human microflora.

J Agric Food Chem. 2003-10-22

[10]
Stevioside and related compounds - molecules of pharmaceutical promise: a critical overview.

Arch Pharm (Weinheim). 2010-11-25

引用本文的文献

[1]
Engineering of cyclodextrin glycosyltransferase improves the conversion efficiency of rebaudioside A to glucosylated steviol glycosides and increases the content of short-chain glycosylated steviol glycoside.

Microb Cell Fact. 2023-6-14

[2]
The Effect of Steviol Glycosides on Sensory Properties and Acceptability of Ice Cream.

Foods. 2022-6-14

[3]
Enrichment of the rebaudioside A concentration in extract with cyclodextrin glycosyltransferase from DSM 13.

Eng Life Sci. 2021-11-10

[4]
Transglycosylation of Steviol Glycosides and Rebaudioside A: Synthesis Optimization, Structural Analysis and Sensory Profiles.

Foods. 2020-11-26

[5]
Differential Cerebral Gustatory Responses to Sucrose, Aspartame, and Stevia Using Gustatory Evoked Potentials in Humans.

Nutrients. 2020-1-27

[6]
Enzymatic Synthesis and Characterization of a Novel α-1→6-Glucosyl Rebaudioside C Derivative Sweetener.

Biomolecules. 2019-1-14

[7]
Nutrient composition of cultivated stevia leaves and the influence of polyphenols and plant pigments on sensory and antioxidant properties of leaf extracts.

J Food Sci Technol. 2010-2-6

[8]
Microwave accelerated transglycosylation of rutin by cyclodextrin glucanotransferase from Bacillus sp. SK13.002.

Int J Mol Sci. 2011

[9]
Engineering of cyclodextrin glucanotransferases and the impact for biotechnological applications.

Appl Microbiol Biotechnol. 2009-9-18

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