Yang G X
Institute of Hepatology, Bethune University of Medical Sciences, Changchun, Jilin.
Zhonghua Zhong Liu Za Zhi. 1991 Mar;13(2):110-3.
Duck hepatitis B virus (DHBV) DNA was detected in both multiple primary cancers and intrahepatic metastatic tumors in duck liver by Southern blot technique. Among 7 ducks with multiple primary tumors in the liver, the banding pattern of integrated DHBV DNA in different tumors was identical in 3 animals but different in 2. One duck showed a similar hybridization pattern in different tumor nodules and the remaining one was negative for DHBV DNA. Integrated DHBV DNA was also identified in one duck with metastatic tumor in the lung, in which the hybridization pattern was the same as that of the primary hepatocellular carcinoma. The presence of the same distinct hybridization bands in various tumor nodules indicated that they may arise from the same clone of neoplastically transformed cell. Different hybridization bands showed that tumors may arise from different clones. The results suggest that hybridization pattern of hepatic carcinoma with viral DNA probe could be used as a clone marker of transformed cells to distinguish multiple primary hepatic cancers from intrahepatic metastasis.
通过Southern印迹技术在鸭肝的多原发性癌和肝内转移瘤中均检测到鸭乙型肝炎病毒（DHBV）DNA。在7只肝脏有多发性原发性肿瘤的鸭中，3只动物不同肿瘤中整合的DHBV DNA的条带模式相同，2只不同。1只鸭在不同肿瘤结节中显示出相似的杂交模式，其余1只鸭的DHBV DNA呈阴性。在1只肺有转移瘤的鸭中也鉴定出整合的DHBV DNA，其杂交模式与原发性肝细胞癌相同。不同肿瘤结节中存在相同的独特杂交带表明它们可能源自同一克隆的肿瘤转化细胞。不同的杂交带表明肿瘤可能源自不同的克隆。结果表明，用病毒DNA探针检测肝癌的杂交模式可作为转化细胞的克隆标记，以区分多原发性肝癌和肝内转移瘤。
