中性粒细胞介导的肿瘤细胞细胞毒性：过氧化物酶系统的作用
Neutrophil-mediated tumor cell cytotoxicity: role of the peroxidase system.
作者信息
Clark R A, Klebanoff S J
出版信息
J Exp Med. 1975 Jun 1;141(6):1442-7. doi: 10.1084/jem.141.6.1442.
Abstract
A cytotoxic effect of human neutrophils on mammalian tumor cells is demonstrated. Cytotoxicity depends on the presence of intact neutrophils, phagocytosable particles, and a halide cofactor and is inhibited by azide, cyanide, and catalase. Neutrophils from patients with myeloperoxidase (MPO) deficiency or defective H1O2 production are not cytotoxic, but activity is resotred by addition of purified MPO or H2O2 respectively. The findings support a mechanism involving the phagocytosis-induced extracellular release of MPO and H2O2 and their reation with a halide cofactor to damage the target cells.
摘要
已证实人类中性粒细胞对哺乳动物肿瘤细胞具有细胞毒性作用。细胞毒性取决于完整中性粒细胞、可吞噬颗粒和卤化物辅助因子的存在，并受到叠氮化物、氰化物和过氧化氢酶的抑制。来自髓过氧化物酶（MPO）缺乏或H2O2产生缺陷患者的中性粒细胞没有细胞毒性，但分别添加纯化的MPO或H2O2可恢复其活性。这些发现支持了一种机制，该机制涉及吞噬作用诱导的MPO和H2O2细胞外释放，以及它们与卤化物辅助因子反应以损伤靶细胞。
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