二氯化三甲基锑在强制摄取后对中国仓鼠卵巢细胞产生遗传毒性作用。
Trimethylantimony dichloride causes genotoxic effects in Chinese hamster ovary cells after forced uptake.
作者信息
Dopp E, Hartmann L M, Florea A-M, von Recklinghausen U, Rabieh S, Shokouhi B, Hirner A V, Rettenmeier A W
机构信息
Institute of Hygiene and Occupational Medicine, University Hospital Essen, Hufelandstrasse 55, 45122 Essen, Germany.
出版信息
Toxicol In Vitro. 2006 Sep;20(6):1060-5. doi: 10.1016/j.tiv.2006.01.018. Epub 2006 Mar 9.
In our study, we demonstrate that trimethylantimony dichloride (TMSb) does not induce micronucleus (MN) formation, chromosome aberrations (CA) or sister chromatid exchanges (SCE) under normal conditions in Chinese hamster ovary (CHO-9) cells in vitro up to an applied concentration of 1 mM, nor is it significantly cytotoxic. TMSb is taken up by the cells in a dose-dependent manner, but the percentage uptake of incubation substrate is low (max 0.05%). Intracellular TMSb concentration is two-fold increased after electroporation and under these forced uptake conditions MN formation is also significantly elevated. These data indicate that resistance to TMSb in CHO-9 cells occurs at the uptake and not at the intracellular level.
在我们的研究中，我们证明了二氯化三甲基锑（TMSb）在体外正常条件下，对中国仓鼠卵巢（CHO-9）细胞施加高达1 mM的浓度时，不会诱导微核（MN）形成、染色体畸变（CA）或姐妹染色单体交换（SCE），也没有明显的细胞毒性。TMSb以剂量依赖的方式被细胞摄取，但孵育底物的摄取百分比很低（最大0.05%）。电穿孔后细胞内TMSb浓度增加了两倍，在这些强制摄取条件下，微核形成也显著升高。这些数据表明，CHO-9细胞对TMSb的抗性发生在摄取水平而非细胞内水平。
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