[三氧化二砷处理多发性骨髓瘤细胞系RPMI 8226后的基因表达谱变化]
[Changes of gene expression profile of multiple myeloma cell line RPMI 8226 treated by arsenic trioxide].
作者信息
Wang Meng-Chang, Liu Shan-Xi, Liu Peng-Bo
机构信息
Department of Hematology, First Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi Province 710061, China.
出版信息
Zhong Xi Yi Jie He Xue Bao. 2006 Mar;4(2):160-5.
OBJECTIVE
To compare the changes of gene expression profiles of multiple myeloma cell line RPMI 8226 before and after 24-hour intervention of arsenic trioxide.
METHODS
The responses of the RPMI 8226 cells to arsenic trioxide were determined with cDNA microarray which included 4,096 different human genes.
RESULTS
Of these 4,096 genes, the expressions of 273 genes were altered significantly at mRNA level. The expressions of 121 genes were up-regulated while the expressions of 152 genes were down-regulated.
CONCLUSION
The effect of arsenic trioxide on RPMI 8226 cells is related to changing the expression levels of a number of genes. ZFYVE16, ALK1 and TXNIP genes may play important roles in apoptosis and differentiation of RPMI 8226 cells.
目的
比较三氧化二砷干预24小时前后多发性骨髓瘤细胞系RPMI 8226基因表达谱的变化。
方法
采用包含4096个人类不同基因的cDNA微阵列检测RPMI 8226细胞对三氧化二砷的反应。
结果
在这4096个基因中，273个基因的表达在mRNA水平有显著改变。其中121个基因表达上调，152个基因表达下调。
结论
三氧化二砷对RPMI 8226细胞的作用与多个基因表达水平的改变有关。ZFYVE16、ALK1和TXNIP基因可能在RPMI 8226细胞的凋亡和分化中起重要作用。
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