Okada Hideki, Fukushi Eri, Yamamori Akira, Kawazoe Naoki, Onodera Shuichi, Kawabata Jun, Shiomi Norio
Ohtaka kohso, Co. Ltd, Otaru 047-0193, Japan.
Carbohydr Res. 2006 May 22;341(7):925-9. doi: 10.1016/j.carres.2006.02.009. Epub 2006 Mar 10.
Fermented beverage of plant extract was prepared from about 50 kinds of vegetables and fruits. Natural fermentation was carried out mainly by lactic acid bacteria (Leuconostoc spp.) and yeast (Zygosaccharomyces spp. and Pichia spp.). Three kinds of saccharides have been found in this beverage and produced by fermentation. The saccharides isolated from the beverage using carbon-Celite column chromatography and preparative HPLC, were identified as a new saccharide, beta-d-fructopyranosyl-(2-->6)-d-glucopyranose, laminaribiose and maltose by examination of constituted sugars, GLC and GC-MS analyses of methyl derivatives and MALDI-TOF-MS and NMR measurements of the saccharides.
植物提取物发酵饮料由约50种蔬菜和水果制成。自然发酵主要由乳酸菌(明串珠菌属)和酵母(接合酵母属和毕赤酵母属)进行。在这种饮料中发现了三种通过发酵产生的糖类。使用碳 - 硅藻土柱色谱和制备型高效液相色谱从饮料中分离出的糖类,通过组成糖类分析、甲基衍生物的气相色谱和气相色谱 - 质谱分析以及糖类的基质辅助激光解吸电离飞行时间质谱和核磁共振测量,被鉴定为一种新的糖类,β - d - 吡喃果糖基 - (2→6) - d - 吡喃葡萄糖、层二糖和麦芽糖。