General Institute of Ohtakakohso, Co., Ltd, Otaru 047-0193, Japan.
Carbohydr Res. 2010 Feb 11;345(3):414-8. doi: 10.1016/j.carres.2009.12.003. Epub 2009 Dec 11.
Four oligosaccharides containing a fructopyranosyl residue have been found from fermented beverage of plant extract and isolated from the beverage using carbon-Celite column chromatography and preparative high performance liquid chromatography. Structure confirmation of the saccharides was provided by methylation analysis, MALDI-TOF-MS and NMR measurements. These saccharides were identified as oligosaccharides of fructopyranoside series; beta-D-fructopyranosyl-(2-->6)-D-fructofuranose (1), beta-D-fructopyranosyl-(2-->1)-D-fructopyranose (2), beta-D-fructopyranosyl-(2-->1)-beta-D-fructofuranosyl-(2<-->1)-alpha-D-glucopyranoside (3), and beta-D-fructopyranosyl-(2-->6)-alpha-D-glucopyranosyl-(1<-->2)-beta-D-fructofuranoside (4). Saccharides 3 and 4 among novel saccharides 1, 3, and 4 were named 'pyrano-1-kestose (pyrano-isokestose)' and 'pyrano-neokestose', respectively.
从植物提取物发酵饮料中发现并分离出 4 种含有果糖残基的低聚糖，采用碳- celite 柱层析和制备高效液相色谱法从饮料中分离出来。通过甲基化分析、MALDI-TOF-MS 和 NMR 测量提供了糖的结构确认。这些糖被鉴定为果糖苷系列的低聚糖；β-D-果糖吡喃糖苷基-(2-- > 6)-D-果糖呋喃糖（1）、β-D-果糖吡喃糖苷基-(2-- > 1)-D-果糖吡喃糖（2）、β-D-果糖吡喃糖苷基-(2-- > 1)-β-D-果糖呋喃糖苷基-(2-- > 1)-α-D-吡喃葡萄糖苷（3）和β-D-果糖吡喃糖苷基-(2-- > 6)-α-D-吡喃葡萄糖基-(1-- > 2)-β-D-果糖呋喃糖苷（4）。新型糖 1、3 和 4 中的糖 3 和 4 分别被命名为“吡喃-1-蔗果三糖（吡喃异蔗果三糖）”和“吡喃-新蔗果三糖”。
