Selvakumar Ponniah, Smith-Windsor Erin, Bonham Keith, Sharma Rajendra K
Department of Pathology and Laboratory of Medicine, College of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E5.
FEBS Lett. 2006 Apr 3;580(8):2021-6. doi: 10.1016/j.febslet.2006.02.076. Epub 2006 Mar 7.
A number of viral and eukaryotic proteins which undergo a lipophilic modification by the enzyme N-myristoyltransferase (NMT: NMT1 and NMT2) are required for signal transduction and regulatory functions. To investigate whether NMT2 contributes to the pathogenesis of colorectal carcinoma, we observed a higher expression of NMT2 in most of the cases of cancerous tissues compared to normal tissues (84.6% of cases; P < 0.05) by Western blot analysis. Furthermore, protein-protein interaction of NMTs revealed that m-calpain interacts with NMT1 while caspase-3 interacts with NMT2. Our findings provide the first evidence of higher expression of NMT2 in human colorectal adenocarcinomas and the interaction of both forms of NMT with various signaling molecules.
许多通过N -肉豆蔻酰转移酶（NMT：NMT1和NMT2）进行亲脂性修饰的病毒和真核蛋白质对于信号转导和调节功能是必需的。为了研究NMT2是否参与结直肠癌的发病机制，我们通过蛋白质免疫印迹分析观察到，与正常组织相比，大多数癌组织病例中NMT2表达更高（84.6%的病例；P < 0.05）。此外，NMTs的蛋白质 - 蛋白质相互作用显示，m - 钙蛋白酶与NMT1相互作用，而半胱天冬酶 - 3与NMT2相互作用。我们的研究结果首次证明了NMT2在人类结直肠腺癌中高表达，以及两种形式的NMT与各种信号分子的相互作用。
