Nok在维持斑马鱼视网膜外核层的完整性方面发挥着重要作用。
Nok plays an essential role in maintaining the integrity of the outer nuclear layer in the zebrafish retina.
作者信息
Wei Xiangyun, Zou Jian, Takechi Masaki, Kawamura Shoji, Li Lihua
机构信息
Department of Ophthalmology, University of Pittsburgh School of Medicine, 203 Lothrop Street, Pittsburgh, PA 15213, USA.
出版信息
Exp Eye Res. 2006 Jul;83(1):31-44. doi: 10.1016/j.exer.2005.10.030. Epub 2006 Mar 10.
Abstract
Proper visual function of the vertebrate retina requires the maintenance of the integrity of the retinal outer nuclear layer (ONL), which is often affected in many blinding human retinal diseases. While the structural integrity of the ONL has long been considered to be maintained primarily through the outer limiting membrane (OLM), we have little knowledge on the development and maintenance of the OLM itself. Here, by analyzing the adhering properties of photoreceptors in zebrafish N-cad and nok mutants, we demonstrated for the first time that the nok gene is essential for the establishment and/or maintenance of the OLM. In addition, our results imply the possibility that Nok, Crumbs, and their associated proteins may constitute a type of photoreceptor-photoreceptor junctional complex that has not be described before. Thus, our study provides novel insights into the mechanisms by which the integrity of the ONL is maintained in the vertebrate retina.
摘要
脊椎动物视网膜的正常视觉功能需要维持视网膜外核层（ONL）的完整性，而在许多致盲性人类视网膜疾病中，ONL常常受到影响。虽然长期以来人们一直认为ONL的结构完整性主要通过外限制膜（OLM）来维持，但我们对OLM本身的发育和维持了解甚少。在这里，通过分析斑马鱼N-cad和nok突变体中光感受器的粘附特性，我们首次证明nok基因对于OLM的建立和/或维持至关重要。此外，我们的结果暗示了Nok、Crumb及其相关蛋白可能构成一种前所未有的光感受器-光感受器连接复合体的可能性。因此，我们的研究为脊椎动物视网膜中ONL完整性的维持机制提供了新的见解。
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