斑马鱼暴露于甲醇会导致视网膜出现模式缺陷和细胞增殖受抑制。
Zebrafish methanol exposure causes patterning defects and suppressive cell proliferation in retina.
作者信息
Fu Jinling, Jiao Jingxue, Weng Kechao, Yu Dan, Li Ruijun
机构信息
Department of Ophthalmology, The Second Hospital of Jilin UniversityChangchun 130041, Jilin, China.
Eye Center of The Second Affiliated Hospital School of Medicine, Institute of Translational Medicine, Zhejiang UniversityHangzhou 310058, Zhejiang, China.
出版信息
Am J Transl Res. 2017 Jun 15;9(6):2975-2983. eCollection 2017.
PURPOSE
Methanol exposure have been shown to produce retinal abnormalities and visual dysfunctions in rodents and other mammals developing . In this study, we characterized how methanol affects the retinal development in an embryonic system, the zebrafish.
METHODS
Zebrafish embryos were raised for 24 hours in fish water supplemented with various concentrations of methanol at 6 hours after fertilisation. The effects of methanol on retinal morphology were assessed by histologic and immunohistochemical analyses.
RESULTS
Zebrafish embryos exposed to moderate (3%) and high (4%) levels of methanol during early embryonic development had a small eye phenotype. Embryos exposed to high (4%) level of methanol had morphological abnormalities of the retinal pigment epithelia and the photoreceptors. Methanol exposure also caused inhibition of cell differentiation and proliferation in the retina at the early developmental stage.
CONCLUSIONS
Low concentrations of methanol affect photoreceptor function but do not disturb retinal morphology. Higher levels of methanol exposure cause retinal patterning defects and a small eye phenotype.
目的
甲醇暴露已被证明会在发育中的啮齿动物和其他哺乳动物中导致视网膜异常和视觉功能障碍。在本研究中，我们描述了甲醇如何影响胚胎系统斑马鱼的视网膜发育。
方法
在受精后6小时，将斑马鱼胚胎在添加了不同浓度甲醇的鱼水中饲养24小时。通过组织学和免疫组织化学分析评估甲醇对视网膜形态的影响。
结果
在胚胎发育早期暴露于中度（3%）和高度（4%）甲醇水平的斑马鱼胚胎出现小眼表型。暴露于高（4%）水平甲醇的胚胎视网膜色素上皮和光感受器存在形态异常。甲醇暴露还导致发育早期视网膜细胞分化和增殖受到抑制。
结论
低浓度甲醇影响光感受器功能，但不干扰视网膜形态。更高水平的甲醇暴露会导致视网膜模式缺陷和小眼表型。
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