与甲醇氧化相关的嗜甲基甲基杆菌AM1的moxF和moxJ基因的核苷酸序列。

Nucleotide sequence of the Methylobacterium extorquens AM1 moxF and moxJ genes involved in methanol oxidation.

作者信息

Anderson D J, Morris C J, Nunn D N, Anthony C, Lidstrom M E

机构信息

Department of Microbiology, University of Washington, Seattle 98195.

出版信息

Gene. 1990 May 31;90(1):173-6. doi: 10.1016/0378-1119(90)90457-3.

DOI:10.1016/0378-1119(90)90457-3

PMID:2116368

Abstract

The nucleotide sequence has been determined for two genes involved in methanol oxidation in the facultative methylotroph, Methylobacterium extorquens AM1. The two genes are moxF, encoding the 66-kDa subunit of the methanol dehydrogenase and moxJ, located immediately downstream from moxF, which encodes a 30-kDa protein with unknown function. This information completes the sequence of the 5.86-kb XhoI-SalI fragment containing the moxFJGI region in M. extorquens AM1, and the structure of this gene cluster is presented. Evidence is presented that moxJ is also present in Paracoccus denitrificans. The aa sequence of MoxJ has provided little information concerning its function, but it does appear to contain a signal sequence suggesting a periplasmic location.

摘要

已确定兼性甲基营养菌嗜甲基生丝微菌AM1中参与甲醇氧化的两个基因的核苷酸序列。这两个基因是moxF，编码甲醇脱氢酶的66 kDa亚基，以及moxJ，位于moxF的紧邻下游，编码一种功能未知的30 kDa蛋白质。这些信息完善了嗜甲基生丝微菌AM1中包含moxFJGI区域的5.86 kb XhoI-SalI片段的序列，并展示了该基因簇的结构。有证据表明反硝化副球菌中也存在moxJ。MoxJ的氨基酸序列几乎没有提供关于其功能的信息，但它似乎确实包含一个信号序列，表明其位于周质。