Duan Likun, Zhao Yan
Department of Chemistry, Iowa State University, Ames, Iowa 50011-3111.
React Funct Polym. 2021 Jan;158. doi: 10.1016/j.reactfunctpolym.2020.104759. Epub 2020 Oct 18.
Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used over-the-counter drugs and their uncontrolled disposal is a significant environmental concern. Although their fluorescent sensing is a desirable method of detection for its sensitivity and simplicity, the structural similarity of the drugs makes the design of selective sensors highly challenging. A thiourea-based fluorescent functional monomer was identified in this work to enable highly efficient synthesis of molecularly imprinted nanoparticle (MINP) sensors for NSAIDs such as Indomethacin or Tolmetin. Micromolar binding affinities were obtained in aqueous solution, with binding selectivities comparable to those reported for polyclonal antibodies. The detection limit was ~50 ng/mL in aqueous solution, and common carboxylic acids such as acetic acid, benzoic acid, and citric acid showed negligible interference.
非甾体抗炎药（NSAIDs）是广泛使用的非处方药，其随意丢弃对环境造成重大影响。尽管基于荧光传感的检测方法因其灵敏度和简便性而备受青睐，但这些药物的结构相似性使得设计选择性传感器极具挑战性。在本研究中，一种基于硫脲的荧光功能单体被用于高效合成用于吲哚美辛或托美汀等非甾体抗炎药的分子印迹纳米颗粒（MINP）传感器。在水溶液中获得了微摩尔级的结合亲和力，结合选择性与多克隆抗体相当。水溶液中的检测限约为50 ng/mL，常见的羧酸如乙酸、苯甲酸和柠檬酸的干扰可忽略不计。
