Techen Natascha, Khan Ikhlas A, Pan Zhiqiang, Scheffler Brian E
National Center for Natural Products Research and Research Institute of Pharmaceutical Sciences, Department of Pharmacognosy, School of Pharmacy, University of Mississippi, USA.
Planta Med. 2006 Feb;72(3):241-7. doi: 10.1055/s-2005-916173.
As part of a continuing research effort to develop chemical and genetic authentication profiles of botanicals, an investigation was performed with the goal to detect, identify and verify Ephedra sinica Stapf DNA in dietary supplements such as plant mixtures and tablets/capsules. We amplified and sequenced the chloroplast psbA-trnH spacer from 21 Ephedra spp. and from two of their closest relatives, Gnetum gnemon L. and Welwitschia mirabilis Hook. Based on sequence comparisons, we identified regions unique to all of the Ephedra spp. samples analyzed. We concluded that the psbA-trnH spacer sequence could be used as a molecular marker. Based on this spacer sequence, we designed Ephedra spp.-specific primers that can help to identify Ephedra spp. DNA in plant mixtures containing as little as 1/1,000 part of Ephedra spp. tissue. We used a DNA extraction method that allows for quick DNA isolation from plant mixtures for PCR analysis.
作为持续开展的开发植物化学和基因鉴定图谱研究工作的一部分，我们进行了一项调查，目的是检测、鉴定和验证膳食补充剂（如植物混合物和片剂/胶囊）中的麻黄草DNA。我们对21种麻黄属植物及其两个近缘物种——马来买麻藤和百岁兰的叶绿体psbA-trnH间隔区进行了扩增和测序。通过序列比较，我们确定了所有分析的麻黄属植物样本特有的区域。我们得出结论，psbA-trnH间隔区序列可作为分子标记。基于该间隔区序列，我们设计了麻黄属植物特异性引物，这些引物有助于鉴定植物混合物中低至千分之一部分麻黄属植物组织的DNA。我们采用了一种DNA提取方法，该方法能够从植物混合物中快速分离DNA用于PCR分析。
