Long Changfeng, Kakiuchi Nobuko, Takahashi Akira, Komatsu Katsuko, Cai Shaoqing, Mikage Masayuki
Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, Japan.
Planta Med. 2004 Nov;70(11):1080-4. doi: 10.1055/s-2004-832651.
Twenty-four Ephedra plants belonging to 8 species grown in the northern and western parts of China were phylogenetically analyzed for their non-coding DNA sequences, internal transcribed spacers (ITSs) of nuclear ribosomal DNA as well as trnL intron and intergenic spacers between trnL and trnF (trnL/ trnF) of the chloroplast. Based on the ITS sequences, the 8 species could be divided into 3 groups: Group 1 (Ephedra intermedia, E. sinica, E. przewalskii), Group 2 (E. equisetina, E. monosperma, E. gerardiana), and Group 3 (E. likiangensis, E. minuta). The species classified into Group 1 grow mainly in the north, Group 3 in the south and Group 2 in the center, suggesting their genetic and geographic relationships. A specific primer set was designed to classify the 3 groups by routine PCR. Combined analysis of ITS and trnL/ trnF differentiated the 8 Ephedra species.
对生长在中国北方和西部的隶属于8个物种的24株麻黄属植物进行了系统发育分析，分析对象包括它们的非编码DNA序列、核糖体DNA的内部转录间隔区（ITS）以及叶绿体的trnL内含子和trnL与trnF之间的基因间隔区（trnL/trnF）。基于ITS序列，这8个物种可分为3组：第1组（中麻黄、草麻黄、膜果麻黄），第2组（木贼麻黄、单子麻黄、藏麻黄），以及第3组（丽江麻黄、矮麻黄）。归入第1组的物种主要生长在北方，第3组生长在南方，第2组生长在中部，这表明了它们的遗传和地理关系。设计了一套特异性引物，通过常规PCR对这3组进行分类。ITS和trnL/trnF的联合分析区分了8种麻黄属植物。
