一种通过海洋浮游植物细胞表面生物素化鉴定的氮调控蛋白的特性。
Characterization of a nitrogen-regulated protein identified by cell surface biotinylation of a marine phytoplankton.
出版信息
Appl Environ Microbiol. 1995 Sep;61(9):3311-5. doi: 10.1128/aem.61.9.3311-3315.1995.
Abstract
The biotinylating reagent succinimidyl 6-(biotinamido)hexanoate was used to label the cell surfaces of the cosmopolitan, marine, eukaryotic microorganism Emiliania huxleyi under different growth conditions. Proteins characteristic of different nutrient conditions could be identified. In particular, a nitrogen-regulated protein, nrp1, has an 82-kDa subunit that is present under nitrogen limitation and during growth on urea. It is absent under phosphate limitation or during exponential growth on nitrate or ammonia. nrp1 is the major membrane or wall protein in nitrogen-limited cells and is found in several strains of E. huxleyi. It may be a useful biomarker for examining the physiological state of E. huxleyi cells in their environment.
摘要
生物素酰化试剂琥珀酰亚胺基 6-（生物胞素酰胺）己酸酯被用于对广布的、海洋的、真核微生物赫氏海豆芽在不同生长条件下的细胞表面进行标记。可以鉴定出具有不同营养条件特征的蛋白质。特别是，一种氮调节蛋白 nrp1，具有一个 82kDa 的亚基，它在氮限制和尿素生长条件下存在，但在磷限制或硝酸盐或氨的指数生长条件下不存在。nrp1 是氮限制细胞中的主要膜或壁蛋白，存在于几种赫氏海豆芽菌株中。它可能是一个有用的生物标志物，用于检查环境中赫氏海豆芽细胞的生理状态。
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