Ralph M. Parsons Laboratory, Department of Civil Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.
Plant Physiol. 1988 May;87(1):143-7. doi: 10.1104/pp.87.1.143.
Plasmalemma redox activity in the diatom Thalassiosira is competitively inhibited by antiserum prepared against algal nitrate reductase (NR), and fluorescent labeling experiments reveal the binding of NR antiserum to the cell surface. Furthermore, the external electron acceptor Cu bathophenanthroline disulfonate causes immediate inhibition of intracellular primary amine production. A model is proposed in which plasmalemma-bound nitrate reductase reduces extracellular electron acceptors and intracellular nitrate and also acts as a trans-plasmalemma proton pump.
硅藻质膜氧化还原活性受到针对藻类硝酸还原酶（NR）的抗血清的竞争性抑制，荧光标记实验显示 NR 抗血清与细胞表面结合。此外，外部电子受体铜二氮杂菲二磺酸会立即抑制细胞内伯胺的产生。提出了一个模型，其中质膜结合的硝酸还原酶还原细胞外电子受体和细胞内的硝酸盐，同时也作为跨质膜质子泵起作用。
