Appl Environ Microbiol. 1995 Dec;61(12):4258-62. doi: 10.1128/aem.61.12.4258-4262.1995.
A bacterial strain which can be grown in a medium containing organic solvents and can secrete a proteolytic enzyme was isolated and identified as Pseudomonas aeruginosa. The strain was derived by the following two-step procedures: high proteolytic enzyme producers were first isolated by the usual method, and then the organic solvent-tolerant microorganism was selected from these high-rate proteolytic enzyme producers. The proteolytic activity of the supernatant of the culture was stable in the presence of various organic solvents. The stability of the enzyme in the presence of organic solvents, of which the values of the logarithm of the partition coefficient (log P) were equal to or more than 3.2, was almost the same as that in the absence of organic solvents. It is expected that both the solvent-tolerant microorganism and the solvent-stable enzyme produced by this strain can be used as catalysts for reactions in the presence of organic solvents.
一种能够在含有有机溶剂的培养基中生长并分泌蛋白水解酶的细菌菌株被分离并鉴定为铜绿假单胞菌。该菌株通过以下两步程序获得：首先通过常规方法分离高产蛋白酶的菌株，然后从这些高产蛋白酶的菌株中选择耐有机溶剂的微生物。培养物上清液中的蛋白水解酶活性在各种有机溶剂存在下稳定。在有机溶剂存在下，其分配系数（log P）值等于或大于 3.2 的酶的稳定性几乎与不存在有机溶剂时相同。预计该菌株产生的耐溶剂微生物和溶剂稳定酶都可以用作有机溶剂存在下反应的催化剂。
