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从烃类污染环境中分离的耐溶剂嗜碱环状芽孢杆菌MTCC 7942产碱性蛋白酶：工艺参数优化

Production of Alkaline Protease by Solvent-Tolerant Alkaliphilic Bacillus circulans MTCC 7942 Isolated from Hydrocarbon Contaminated Habitat: Process Parameters Optimization.

作者信息

Patil Ulhas, Chaudhari Ambalal

机构信息

Department of Microbiology, R. C. Patel Arts, Commerce and Science College, Shirpur 425 405, India.

School of Life Sciences, North Maharashtra University, Jalgaon 425 001, India.

出版信息

ISRN Biochem. 2013 Nov 28;2013:942590. doi: 10.1155/2013/942590. eCollection 2013.

DOI:10.1155/2013/942590
PMID:25937965
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4392993/
Abstract

In the present investigation, a newly isolated organic solvent-tolerant and alkaliphilic bacterial strain was reported from a hydrocarbon (gasoline and diesel) contaminated soil collected from the petrol station, Shirpur (India). The strain was identified as Bacillus circulans MTCC 7942, based on phenotype, biochemical, and phylogenetic analysis of 16S rRNA gene sequence. The capability of Bacillus circulans to secrete an extracellular, thermostable, alkaline protease and grow in the presence of organic solvents was explored. Bacillus circulans produced maximum alkaline protease (412 U/mL) in optimized medium (g/L): soybean meal, 15; starch, 10; KH2PO4, 1; MgSO4·7H2O, 0.05; CaCl2, 1; Na2CO3, 8; pH 10.0 at 37°C and 100 rpm. The competence of strain to grow in various organic solvents-n-octane, dodecane, n-decane, N,N-dimethylformamide, n-hexane, and dimethyl sulfoxide, establishes its potential as solvent-stable protease source for the possible applications in nonaqueous reactions and fine chemical synthesis.

摘要

在本研究中，从印度希尔布尔加油站受碳氢化合物（汽油和柴油）污染的土壤中分离出了一种新的耐有机溶剂嗜碱细菌菌株。基于16S rRNA基因序列的表型、生化和系统发育分析，该菌株被鉴定为环状芽孢杆菌MTCC 7942。研究了环状芽孢杆菌分泌细胞外耐热碱性蛋白酶以及在有机溶剂存在下生长的能力。环状芽孢杆菌在优化培养基（g/L）中产生的碱性蛋白酶最多（412 U/mL）：豆粕15、淀粉10、KH2PO4 1、MgSO4·7H2O 0.05、CaCl2 1、Na2CO3 8，在37°C和100 rpm条件下pH为10.0。该菌株在各种有机溶剂（正辛烷、十二烷、正癸烷、N，N-二甲基甲酰胺、正己烷和二甲基亚砜）中生长的能力，确立了其作为溶剂稳定蛋白酶来源在非水反应和精细化学合成中可能应用的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7408/4392993/41b24b55ceba/ISRN.BIOCHEMISTRY2013-942590.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7408/4392993/ea6ba0535d2f/ISRN.BIOCHEMISTRY2013-942590.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7408/4392993/09afc079a7b4/ISRN.BIOCHEMISTRY2013-942590.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7408/4392993/ba32556d86af/ISRN.BIOCHEMISTRY2013-942590.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7408/4392993/4b57021afe0d/ISRN.BIOCHEMISTRY2013-942590.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7408/4392993/1c5b7b494dcb/ISRN.BIOCHEMISTRY2013-942590.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7408/4392993/41b24b55ceba/ISRN.BIOCHEMISTRY2013-942590.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7408/4392993/ea6ba0535d2f/ISRN.BIOCHEMISTRY2013-942590.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7408/4392993/09afc079a7b4/ISRN.BIOCHEMISTRY2013-942590.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7408/4392993/ba32556d86af/ISRN.BIOCHEMISTRY2013-942590.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7408/4392993/4b57021afe0d/ISRN.BIOCHEMISTRY2013-942590.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7408/4392993/1c5b7b494dcb/ISRN.BIOCHEMISTRY2013-942590.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7408/4392993/41b24b55ceba/ISRN.BIOCHEMISTRY2013-942590.006.jpg

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