Appl Environ Microbiol. 1995 Dec;61(12):4278-83. doi: 10.1128/aem.61.12.4278-4283.1995.
Addition of nitrapyrin, allylthiourea, C(inf2)H(inf2), and CH(inf3)F to freshwater sediment slurries inhibited CH(inf4) oxidation and nitrification to similar extents. Dicyandiamide and allylsulfide were less inhibitory for CH(inf4) oxidation than for nitrification. Allylsulfide was the most potent inhibitor of nitrification, and the estimated 50% inhibitory concentrations for this process and CH(inf4) oxidation were 0.2 and 121 (mu)M, respectively. At a concentration of 2 (mu)M allylsulfide, growth and CH(inf4) oxidation activity of Methylosinus trichosporium OB3b were not inhibited. Allylsulfide at 200 (mu)M inhibited the growth of M. trichosporium by approximately 50% but did not inhibit CH(inf4) oxidation activity. Nitrite production by cells of M. trichosporium was not significantly affected by allylsulfide, except at a concentration of 2 mM, when growth and CH(inf4) oxidation were also inhibited by about 50%. Methane monooxygenase activity present in soluble fractions of M. trichosporium was not inhibited significantly by allylsulfide at either 200 (mu)M or 2 mM. These results suggest that the partial inhibition of CH(inf4) oxidation in sediment slurries by high allylsulfide concentrations may be caused by an inhibition of the growth of methanotrophs rather than an inhibition of methane monooxygenase activity specifically. We conclude that allylsulfide is a promising tool for the study of interactions of methanotrophs and nitrifiers in N cycling and CH(inf4) turnover in natural systems.
向淡水沉积物悬浮液中添加硝普盐、烯丙基硫脲、C(inf2)H(inf2)和 CH(inf3)F 会抑制 CH(inf4)氧化和硝化作用，抑制程度相似。双氰胺和烯丙基硫脲对 CH(inf4)氧化的抑制作用比对硝化作用的抑制作用小。烯丙基硫脲是硝化作用的最有效抑制剂，对该过程和 CH(inf4)氧化的估计 50%抑制浓度分别为 0.2 和 121（mu）M。在 2（mu）M 烯丙基硫脲浓度下，Methylosinus trichosporium OB3b 的生长和 CH(inf4)氧化活性不受抑制。200（mu）M 烯丙基硫脲抑制 M. trichosporium 的生长约 50%，但不抑制 CH(inf4)氧化活性。烯丙基硫脲对 M. trichosporium 细胞的亚硝酸盐生成没有明显影响，除了在 2 mM 浓度下，此时生长和 CH(inf4)氧化也被抑制约 50%。M. trichosporium 可溶性部分的甲烷单加氧酶活性在 200（mu）M 或 2 mM 时均未被烯丙基硫脲显著抑制。这些结果表明，高浓度烯丙基硫脲对沉积物悬浮液中 CH(inf4)氧化的部分抑制可能是由于甲烷营养菌生长受到抑制，而不是甲烷单加氧酶活性受到特异性抑制所致。我们得出结论，烯丙基硫脲是研究自然系统中氮循环和 CH(inf4)周转中甲烷营养菌和硝化菌相互作用的一种很有前途的工具。
