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Appl Environ Microbiol. 1984 Feb;47(2):258-62. doi: 10.1128/aem.47.2.258-262.1984.
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Inhibitory effect of nitrapyrin on three genera of ammonia-oxidizing nitrifiers.硝吡咯菌素对三属氨氧化硝化细菌的抑制作用。
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THE EFFECT OF 2-CHLORO, 6-(TRICHLOROMETHYL) PYRIDINE ON THE CHEMOAUTOTROPHIC METABOLISM OF NITRIFYING BACTERIA. II. NITRITE OXIDATION BY NITROBACTER.2-氯-6-（三氯甲基）吡啶对硝化细菌化学自养代谢的影响。II. 硝化杆菌对亚硝酸盐的氧化作用
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[Comparative studies of quantitative determination of proteins in cerebrospinal fluid and low-protein solutions].[脑脊液和低蛋白溶液中蛋白质定量测定的比较研究]
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The biochemistry of the nitrifying organisms. IV. The respiration and intermediary metabolism of Nitrosomonas.硝化生物的生物化学。IV. 亚硝化单胞菌的呼吸作用与中间代谢
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Production of nitrite from ammonia by methane oxidizing bacteria.甲烷氧化细菌将氨转化为亚硝酸盐。
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Enrichment, isolation and some properties of methane-utilizing bacteria.甲烷利用菌的富集、分离及某些特性
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Effect of chemicals used as nitrification inhibitors on the denitrification process.用作硝化抑制剂的化学物质对反硝化过程的影响。
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硝吡咯菌素[2-氯-6-(三氯甲基)吡啶]对严格甲烷营养菌 Methylosinus trichosporium OB3b 的影响。

Effects of Nitrapyrin [2-Chloro-6-(Trichloromethyl) Pyridine] on the Obligate Methanotroph Methylosinus trichosporium OB3b.

机构信息

Department of Microbiology, Macdonald College of McGill University, Ste. Anne de Bellevue, Quebec H9X 1C0, Canada.

出版信息

Appl Environ Microbiol. 1984 Feb;47(2):258-62. doi: 10.1128/aem.47.2.258-262.1984.

DOI:10.1128/aem.47.2.258-262.1984
PMID:16346465
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC239655/
Abstract

Nitrapyrin inhibited growth, CH(4) oxidation, and NH(4) oxidation, but not the oxidation of CH(3)OH, HCHO, or HCOONa, by Methylosinus trichosporium OB3b, suggesting that nitrapyrin acts against the methane monooxygenase enzyme system. The inhibition of CH(4) oxidation could be reversed by repeated washing of nitrapyrin-inhibited cells, indicating that its effect is bacteriostatic. The addition of Cu did not release the inhibition. Methane oxidation was also inhibited by 6-chloro-2-picoline. These data suggest that the mode of action of nitrapyrin on M. trichosporium is different from that on chemoautotrophic NH(4) oxidizers or methanogens.

摘要

硝吡咯菌素抑制了 Methylosinus trichosporium OB3b 的生长、CH(4)氧化、NH(4)氧化，但不抑制 CH(3)OH、HCHO 或 HCOONa 的氧化，表明硝吡咯菌素作用于甲烷单加氧酶酶系统。硝吡咯菌素抑制的细胞经反复洗涤后，CH(4)氧化的抑制作用可以逆转，表明其作用是抑菌作用。添加 Cu 并不能解除抑制作用。6-氯-2-吡啶甲酸也抑制甲烷氧化。这些数据表明，硝吡咯菌素对 M. trichosporium 的作用方式不同于对化能自养 NH(4)氧化菌或产甲烷菌的作用方式。