Appl Environ Microbiol. 1996 Mar;62(3):1051-7. doi: 10.1128/aem.62.3.1051-1057.1996.
A turbidimetric technique was used to measure the bacteriolytic activities of digestive fluids collected seasonally from the deposit-feeding polychaete Arenicola marina. Lysis of cultured sedimentary bacteria was monitored spectrophotometrically. The kinetics of the lytic reaction were characterized with respect to temperature, pH, reactant concentration, and time. Bacteriolysis generally followed saturation kinetics behavior and was apparently a first-order process with respect to the substrate (bacteria). Deviation from a simple Michaelis-Menten relationship, however, was observed at high substrate levels, at which inhibition of lysis was evident. The lytic rate decreased rapidly and sharply through time as the substrate was depleted. Lytic factors in the gut fluid acted as catalysts; rates increased with concentration of gut fluid, but no depletion in lytic capacity through time was observed. The lytic rate increased exponentially for all seasonal samples over the range of environmental temperatures (4 to 31(deg)C) but showed little relationship with pH under conditions likely to be experienced in situ. The lytic rate also varied among bacterial strains and with culture age.
采用比浊法测定了季节收集的食沉积物多毛环节虫 Arenicola marina 的消化液的溶菌活性。通过分光光度法监测培养沉积物细菌的裂解情况。从温度、pH 值、反应物浓度和时间等方面对溶菌反应动力学进行了研究。溶菌作用通常遵循饱和动力学行为，并且显然是底物（细菌）的一级过程。然而，在高底物水平下观察到与简单米氏-门坦关系的偏离，此时明显存在溶菌抑制作用。随着底物的消耗，溶菌速率随时间迅速而急剧下降。肠道液中的溶菌因子作为催化剂；随着肠道液浓度的增加，速率增加，但在整个时间内没有观察到溶菌能力的消耗。在所有季节性样本中，溶菌速率在环境温度范围内（4 至 31°C）呈指数增长，但在原位可能遇到的条件下与 pH 值几乎没有关系。溶菌速率也因菌株和培养年龄而异。
