Arvidson S, Holme T, Wadström T
J Bacteriol. 1970 Oct;104(1):227-33. doi: 10.1128/jb.104.1.227-233.1970.
The formation of bacteriolytic enzymes of Staphylococcus aureus, with special reference to strain M18, was investigated under a variety of conditions. The bacteriolytic activity was tested by using whole cells of Micrococcus lysodeikticus as a substrate. Complex media were required for production, and a Casein Hydrolysate-Yeast Extract medium (CCY(I)) was superior to Brain Heart Infusion and Trypticase Soy Broth. The optimal pH level for production was 7.0. Effective oxygenation and exchange of the beta-glycerophosphate of the CCY(I) medium for glucose increased the rates of growth and autolysis and the rate of appearance of extracellular bacteriolytic enzymes. However, the extracellular lytic activity decreased more rapidly at the end of the growth period than under the standard culture conditions. The appearance of inhibitor(s), probably derived from autolysis, might be responsible for this rapid decrease. The highest yields were obtained in a continuous process in which the activity was almost twice that of batch cultures grown under the same conditions. The bacteriolytic activity produced in continuous culture had a considerably increased stability in the purification process. The advantage of producing unstable bacterial proteins in continuous culture under controlled growth conditions is discussed.
在多种条件下研究了金黄色葡萄球菌溶菌酶的形成，特别提及了M18菌株。以溶壁微球菌的全细胞为底物检测溶菌活性。生产需要复杂培养基，酪蛋白水解物 - 酵母提取物培养基（CCY(I)）优于脑心浸液和胰蛋白胨大豆肉汤。生产的最适pH值为7.0。CCY(I)培养基中β - 甘油磷酸酯有效充氧并换成葡萄糖可提高生长和自溶速率以及细胞外溶菌酶的出现速率。然而，与标准培养条件相比，生长末期细胞外溶菌活性下降得更快。可能源自自溶的抑制剂的出现可能是导致这种快速下降的原因。在连续培养过程中获得了最高产量，其活性几乎是在相同条件下分批培养的两倍。连续培养产生的溶菌活性在纯化过程中的稳定性显著提高。讨论了在受控生长条件下连续培养生产不稳定细菌蛋白的优势。
