Appl Environ Microbiol. 1996 Oct;62(10):3878-80. doi: 10.1128/aem.62.10.3878-3880.1996.
Sucrose uptake by Corynebacterium glutamicum involves a phosphoenolpyruvate-dependent sucrose phosphotransferase (PTS), but in the absence of fructokinase, further metabolism of the liberated fructose requires efflux of the fructose and reassimilation via the fructose PTS. Mutant strains lacking detectable fructose-transporting PTS activity accumulated fructose extracellularly but consumed sucrose at rates comparable to those of the wild-type strain.
谷氨酸棒杆菌摄取蔗糖涉及依赖磷酸烯醇丙酮酸的蔗糖磷酸转移酶(PTS),但在没有果糖激酶的情况下,释放的果糖的进一步代谢需要果糖通过果糖 PTS 外排和再摄取。缺乏可检测果糖转运 PTS 活性的突变株在外周积累果糖,但消耗蔗糖的速度与野生型菌株相当。