Appl Environ Microbiol. 1997 Mar;63(3):1171-4. doi: 10.1128/aem.63.3.1171-1174.1997.
A microbial culture enriched from a diesel fuel-contaminated aquifer was able to grow on 1,3,5-trimethylbenzene (1,3,5-TMB) and 1,2,4-TMB under N(inf2)O-reducing conditions, but it did not degrade 1,2,3-TMB. The oxidation of 1,3,5-TMB to CO(inf2) was coupled to the production of biomass and the reduction of N(inf2)O. N(inf2)O was used to avoid toxic effects caused by NO(inf2)(sup-) accumulation during growth with NO(inf3)(sup-) as the electron acceptor. In addition to 1,3,5-TMB and 1,2,4-TMB, the culture degraded toluene, m-xylene, p-xylene, 3-ethyltoluene, and 4-ethyltoluene.
从受柴油污染的含水层中富集的微生物培养物能够在 N₂O 还原条件下生长 1,3,5-三甲基苯(1,3,5-TMB)和 1,2,4-TMB,但不能降解 1,2,3-TMB。1,3,5-TMB 的氧化与生物量的产生和 N₂O 的还原偶联。使用 N₂O 来避免在以 NO₃⁻作为电子受体生长过程中由于 NO₂⁻积累而引起的毒性作用。除了 1,3,5-TMB 和 1,2,4-TMB,该培养物还降解甲苯、间二甲苯、对二甲苯、3-乙基甲苯和 4-乙基甲苯。