Appl Environ Microbiol. 1997 Aug;63(8):3164-9. doi: 10.1128/aem.63.8.3164-3169.1997.
Petroleum contains many heterocyclic organosulfur compounds refractory to conventional hydrodesulfurization carried out with chemical catalysts. Among these, dibenzothiophene (DBT) and DBTs bearing alkyl substitutions are representative compounds. Two bacterial strains, which have been identified as Paenibacillus strains and which are capable of efficiently cleaving carbon-sulfur (C--S) bonds in DBT at high temperatures, have been isolated for the first time. Upon attacking DBT and its various methylated derivatives at temperatures up to 60(deg)C, both growing and resting cells of these bacteria can release sulfur atoms as sulfate ions and leave the monohydroxylated hydrocarbon moieties intact. Moreover, when either of these paenibacilli was incubated at 50(deg)C with light gas oil previously processed through hydrodesulfurization, the total sulfur content in the oil phase clearly decreased.
石油中含有许多杂环有机含硫化合物，这些化合物难以用化学催化剂进行常规加氢脱硫处理。其中，二苯并噻吩（DBT）和带有烷基取代基的 DBT 是代表性化合物。首次分离出了两种能够在高温下有效切断 DBT 中碳-硫（C-S）键的细菌菌株，它们被鉴定为芽孢杆菌属菌株。这两种细菌在温度高达 60°C 的情况下攻击 DBT 及其各种甲基衍生物时，无论是生长细胞还是休眠细胞都可以将硫原子释放为硫酸根离子，而使单羟基化的烃基部分保持完整。此外，当其中任何一种芽孢杆菌在 50°C 与经过加氢脱硫处理的轻质瓦斯油一起孵育时，油相中总硫含量明显下降。
