硫酸盐还原菌对间甲酚的厌氧降解。
Anaerobic Degradation of m-Cresol by a Sulfate-Reducing Bacterium.
出版信息
Appl Environ Microbiol. 1997 Aug;63(8):3170-5. doi: 10.1128/aem.63.8.3170-3175.1997.
m-Cresol metabolism under sulfate-reducing conditions was studied with a pure culture of Desulfotomaculum sp. strain Groll. Previous studies with a sulfate-reducing consortium indicated that m-cresol was degraded via an initial para-carboxylation reaction. However, 4-hydroxy-2-methylbenzoic acid was not degraded by strain Groll, and no evidence for ring carboxylation of m-cresol was found. Strain Groll readily metabolized the putative metabolites of a methyl group oxidation pathway, including 3-hydroxybenzyl alcohol, 3-hydroxybenzaldehyde, 3-hydroxybenzoic acid, and benzoic acid. Degradation of these compounds preceded and inhibited m-cresol decay. 3-Hydroxybenzoic acid was detected in cultures that received either m-cresol or 3-hydroxybenzyl alcohol, and trace amounts of benzoic acid were detected in m-cresol-degrading cultures. Therefore, we propose that strain Groll metabolizes m-cresol by a methyl group oxidation pathway which is an alternate route for the catabolism of this compound under sulfate-reducing conditions.
在硫酸盐还原条件下，利用脱硫单胞菌（Desulfotomaculum sp.）菌株 Groll 的纯培养物研究了间甲酚的代谢。先前对硫酸盐还原共生物的研究表明，间甲酚通过初始对位羧化反应降解。然而，菌株 Groll 不能降解 4-羟基-2-甲基苯甲酸，也没有发现间甲酚环羧化的证据。菌株 Groll 很容易代谢甲基氧化途径的假定代谢物，包括 3-羟基苯甲醇、3-羟基苯甲醛、3-羟基苯甲酸和苯甲酸。这些化合物的降解先于并抑制了间甲酚的衰减。在接受间甲酚或 3-羟基苯甲醇的培养物中检测到 3-羟基苯甲酸，并且在降解间甲酚的培养物中检测到痕量苯甲酸。因此，我们提出菌株 Groll 通过甲基氧化途径代谢间甲酚，这是该化合物在硫酸盐还原条件下分解的替代途径。
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