Rabus R, Widdel F
Max-Planck-Institut für Marine Mikrobiologie, Fahrenheitstrasse 1, D-28359 Bremen, Germany.
Arch Microbiol. 1995 Dec;164(6):448-51. doi: 10.1007/BF02529744.
Anaerobic toluene oxidation by the sulfate-reducing bacterium, strain Tol2 (proposed name Desulfobacula toluolica) was specifically inhibited by benzyl alcohol when added at concentrations around 500 microM. Benzyl alcohol added at lower, non-inhibitory concentrations (around 5 microM) was not oxidized by active cells pregrown on toluene, indicating that the alcohol is not a free intermediate of toluene metabolism in the sulfate reducer. Conversion of p-xylene in toluene-metabolizing cells to p-methylbenzoate as dead-end product suggests that the sulfate reducer, like denitrifiers, initiates toluene oxidation at the methyl group.
当以约500微摩尔的浓度添加时，硫酸盐还原菌Tol2菌株（提议命名为甲苯脱硫杆菌）对甲苯的厌氧氧化受到苯甲醇的特异性抑制。以较低的、非抑制性浓度（约5微摩尔）添加的苯甲醇不会被在甲苯上预培养的活性细胞氧化，这表明该醇不是硫酸盐还原菌中甲苯代谢的游离中间体。在甲苯代谢细胞中将对二甲苯转化为对甲基苯甲酸作为终产物表明，硫酸盐还原菌与反硝化菌一样，在甲基处启动甲苯氧化。
