Schennen U, Braun K, Knackmuss H J
J Bacteriol. 1985 Jan;161(1):321-5. doi: 10.1128/jb.161.1.321-325.1985.
Three strains of anaerobically benzoate-degrading, denitrifying bacteria of the genus Pseudomonas were able to grow on 2-fluorobenzoate as the sole carbon and energy source. Fluoride ion release was stoichiometric, and the reduction of dissolved organic carbon indicated total degradation. Cells grown anaerobically with benzoate were adapted for immediate growth with 2-fluorobenzoate, and both compounds were substrates for an inducible benzoyl-coenzyme A synthetase, the initial enzyme of anaerobic degradation. It is proposed that fluoride is eliminated gratuitously by a regioselective reaction in a sequence common to both carbon sources. Benzoate, but not 2-fluorobenzoate, was oxidized by aerobically grown cells.
三株厌氧降解苯甲酸盐的反硝化假单胞菌能够以2-氟苯甲酸盐作为唯一碳源和能源生长。氟离子的释放是化学计量的,溶解有机碳的减少表明完全降解。以苯甲酸盐厌氧培养的细胞能够适应以2-氟苯甲酸盐立即生长,并且这两种化合物都是诱导型苯甲酰辅酶A合成酶(厌氧降解的初始酶)的底物。有人提出,在两种碳源共有的序列中,通过区域选择性反应可无因次地消除氟化物。需氧生长的细胞可氧化苯甲酸盐,但不能氧化2-氟苯甲酸盐。