Appl Environ Microbiol. 1997 Sep;63(9):3729-31. doi: 10.1128/aem.63.9.3729-3731.1997.
Permeabilized cells of toluene-mineralizing, sulfate-reducing strain PRTOL1 catalyzed the addition of toluene to fumarate to form benzylsuccinate under anaerobic conditions. Recent in vitro studies with two toluene-mineralizing, denitrifying bacteria demonstrated the same fumarate addition reaction and indicated that it may be the first step of anaerobic toluene degradation. This study with strain PRTOL1 shows that anaerobic toluene activation by fumarate addition occurs in bacteria as disparate as sulfate-reducing and denitrifying species (members of the delta and beta subclasses of the Proteobacteria, respectively).
经甲苯矿化、硫酸盐还原菌株 PRTOL1 通透化处理的细胞在厌氧条件下催化甲苯与富马酸加成生成苯丁烯二酸。最近的两项甲苯矿化、反硝化细菌的体外研究表明了相同的富马酸加成反应,并表明它可能是厌氧甲苯降解的第一步。本研究用菌株 PRTOL1 表明,反硝化和硫酸盐还原等不同种类的细菌(分别属于变形菌的 δ 和 β 亚类)都能通过富马酸加成来实现厌氧甲苯的激活。