Field J B
Metabolism. 1975 Mar;24(3):381-93. doi: 10.1016/0026-0495(75)90118-3.
The initial step in TSH action reflects binding of the hormone to specific receptor sites on the plasma membrane. Such binding has been studied using plasma membranes, homogenates, isolated thyroid cells grown in culture, and thyroid slices. 3-H- and iodinated TSH preparations have been used; the latter have been prepared using both chloramine-T and lactoperoxidase. Some of the discrepancies reported in the literature might reflect the different thyroid and hormone preparations and the variable incubation conditions which have been used. In general, good correlation exists between binding of TSH and activation of adenylate cyclase in thyroid plasma membranes. Data is reviewed related to activation of protein kinase in intact thyroid cells by TSH. Although there is impressive evidence for cyclic AMP mediation of effects of TSH on the thyroid, some data that are inconsistent with this concept are considered, especially in relationship to 32-P incorporation into phospholipid. The role of cyclic GMP in thyroid function is discussed.
促甲状腺激素（TSH）作用的初始步骤反映了该激素与质膜上特定受体位点的结合。这种结合已使用质膜、匀浆、培养的分离甲状腺细胞以及甲状腺切片进行了研究。已使用³H标记和碘化的TSH制剂；后者是使用氯胺 - T和乳过氧化物酶制备的。文献中报道的一些差异可能反映了所使用的不同甲状腺和激素制剂以及可变的孵育条件。一般来说，TSH的结合与甲状腺质膜中腺苷酸环化酶的激活之间存在良好的相关性。本文综述了与TSH在完整甲状腺细胞中激活蛋白激酶相关的数据。尽管有令人信服的证据表明TSH对甲状腺的作用是由环磷酸腺苷（cAMP）介导的，但也考虑了一些与此概念不一致的数据，特别是与³²P掺入磷脂有关的数据。还讨论了环磷酸鸟苷（cGMP）在甲状腺功能中的作用。
