[兔肿瘤细胞系VX2的建立与鉴定]

[Establishment and characterization of a rabbit tumor cell line VX2].

作者信息

Liu Xian-feng, Ren Le-rong, Su Guang-yan, Liu Yu-qin, Gu Bei, Dong Ji-hong

机构信息

Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.

出版信息

Zhonghua Bing Li Xue Za Zhi. 2005 Oct;34(10):661-3.

PMID:16536280

Abstract

OBJECTIVE

To establish a rabbit tumor cell line and to characterize its biological parameters.

METHODS

VX2 tumor tissue was used for the primary culture in vitro. After 40 passages, the cell morphology, CK expression (immunohistochemical staining), cell cycle, karyotype and tumorigenecity in rabbits and nude mice were investigated.

RESULTS

The newly established cell line VX2 was maintained in continuous culture for over 70 passages in 10 months. Morphologically, VX2 cells were polygonal to short spindled. Tonal fibril and tight junction were found under the electron microscope. CK was positive. The cell cycle analysis showed 69.3% in G1 phase, 5.6% in G2 phase and 25.1% in S phase. The population doubling time was 34.5 hours. The chromosomal analysis showed a hypotriploidy with a median chromosome number of 58 approximately 62. The tumorigenecity in rabbits and nude mice were both 100%.

CONCLUSION

The established VX2 cell line derived from rabbit squamous carcinoma could serve as a model system for experimental oncology in the rabbit.

摘要

目的

建立兔肿瘤细胞系并对其生物学参数进行表征。

方法

采用VX2肿瘤组织进行体外原代培养。传代40次后，对细胞形态、细胞角蛋白（CK）表达（免疫组织化学染色）、细胞周期、核型以及在兔和裸鼠体内的致瘤性进行研究。

结果

新建立的VX2细胞系在10个月内连续传代70余次。形态学上，VX2细胞呈多边形至短梭形。电镜下可见张力原纤维和紧密连接。CK呈阳性。细胞周期分析显示，G1期占69.3%，G2期占5.6%，S期占25.1%。群体倍增时间为34.5小时。染色体分析显示为亚三倍体，中位数染色体数约为58至62条。在兔和裸鼠体内的致瘤性均为100%。