Harada M, Miyata K, Wada T, Morita N, Sakamoto T
Department of Dentistry and Oral Surgery, Wakayama Medical College, Wakayama, Japan.
Hum Cell. 1993 Mar;6(1):29-35.
We established a cell line (H-1) from human oral cancer. It was derived from a biopsy specimen of squamous cell carcinoma in the lower gingiva of a japanese man. The cell line has now been growing in vitro for more than 86 passages. The cells had an epithelial morphology and grew as adherent monolayers. PAS stain was positive and activity of ALP also was positive. They had a doubling time of 37 hours and colony formation of 32%. Chromosomal numbers ranged from 49 to 75 and the modal number was 61. The cell line was tumorigenic in nude mice, its histologic examination showed identification with that from the original tumor. The cells were sensitive to hyperthermia.
我们从人类口腔癌中建立了一个细胞系（H-1）。它源自一名日本男性下牙龈鳞状细胞癌的活检标本。该细胞系现已在体外培养超过86代。细胞具有上皮形态，以贴壁单层生长。PAS染色呈阳性，碱性磷酸酶活性也呈阳性。它们的倍增时间为37小时，集落形成率为32%。染色体数目在49至75之间，众数为61。该细胞系在裸鼠中具有致瘤性，其组织学检查显示与原发肿瘤一致。这些细胞对热疗敏感。
