Li S Y, Skinner A C
Natural Resources Canada, Canadian Forest Service, Atlantic Forestry Centre, P.O. Box 960, University Drive, Corner Brook, Newfoundland and Labrador, Canada A2H 6J3.
J Econ Entomol. 2005 Dec;98(6):1876-9. doi: 10.1093/jee/98.6.1876.
Virus yield produced by dead larvae of balsam fir sawfly, Neodiprion abietis (Harris) (Hymenoptera: Diprionidae), that had been infected at four different larval stages (second, third, fourth, or fifth instar) with two virus concentrations (10(5) polyhedral inclusion bodies (PIB) /ml or 10(7) PIB/ml), were analyzed and compared to determine the effects of instar and amount of virus inoculum on virus production. The results indicate that both larval stage and inoculation dosage significantly affect virus yield. On average, each dead larva produced 1.36-12.21 x 10(7) PIB, depending upon larval age and virus concentration of inoculation. Although each dead larva produced more PIB when it was inoculated in the fourth or fifth stage, inoculation of these larvae did not result in the highest virus yield because of low larval mortality. In terms of net virus return, third instars would maximize virus yield when they are inoculated with a virus concentration that can cause 95-100% larval mortality.
分析并比较了被两种病毒浓度（10⁵ 个多角体包涵体（PIB）/毫升或 10⁷ PIB/毫升）感染的香脂冷杉叶蜂（Neodiprion abietis (Harris)，膜翅目：叶蜂科）在四个不同幼虫阶段（二龄、三龄、四龄或五龄）死亡幼虫产生的病毒产量，以确定幼虫龄期和病毒接种量对病毒产生的影响。结果表明，幼虫龄期和接种剂量均显著影响病毒产量。平均而言，根据幼虫年龄和接种的病毒浓度，每只死亡幼虫产生 1.36 - 12.21×10⁷ 个 PIB。虽然在四龄或五龄接种时，每只死亡幼虫产生的 PIB 更多，但由于幼虫死亡率低，接种这些幼虫并未产生最高的病毒产量。就净病毒回报而言，当三龄幼虫接种能导致 95 - 100%幼虫死亡的病毒浓度时，病毒产量将最大化。
