Graves Roger, Lucarotti Christopher J, Quiring Dan T
Faculty of Forestry and Environmental Management, The University of New Brunswick, Fredericton, New Brunswick E3B 5A3, Canada.
Natural Resources Canada, Canadian Forest Service-Atlantic Forestry Centre, 1350 Regent Street, Fredericton, New Brunswick E3C 2G6, Canada.
Insects. 2012 Sep 28;3(4):912-29. doi: 10.3390/insects3040912.
Field trials and assessments of the balsam fir sawfly (Neodiprion abietis) nucleopolyhedrovirus (NeabNPV: Baculoviridae, Gammabaculovirus) against its natural host were conducted in July and August 2002 near Corner Brook, Newfoundland and Labrador, Canada, in naturally regenerated, precommercially thinned stands dominated by balsam fir (Abies balsamea). Two experimental blocks, each with its own untreated control, were established. The purpose of the Island Pond block was to examine the spread of NeabNPV from a 313-ha aerial treatment block out into adjacent populations of balsam fir sawflies. The purpose of the Old Man's Pond block (2,093 ha) was to determine whether NeabNPV could disperse into populations of balsam fir sawflies within a 200-m zone between spray swaths. NeabNPV was applied to treatment blocks by a Cessna 188B AgTruck aircraft equipped with MicronAir AU4000 rotary atomizers at an application rate equivalent to 1 × 10⁸ NeabNPV occlusion bodies/ha in 2.5 L of 20% aqueous molasses. At Island Pond, NeabNPV infection increased with time following the spray, especially for individuals close to the treatment block, and infection rate decreased to a measured distance of 400 m from the treatment block. At Old Man's Pond, NeabNPV infection rose higher (80% vs. 15%) and sawfly densities declined more (84% vs. 60%) in the area between spray swaths than in the control block.
2002年7月和8月，在加拿大纽芬兰与拉布拉多省科纳布鲁克附近，于天然更新、处于预商业疏伐阶段且以香脂冷杉（Abies balsamea）为主的林分中，开展了针对香脂冷杉锯角叶蜂（Neodiprion abietis）核多角体病毒（NeabNPV：杆状病毒科，γ杆状病毒属）对其天然寄主的田间试验和评估。设立了两个试验区，每个试验区都有各自的未处理对照区。岛塘试验区的目的是研究NeabNPV从一个313公顷的空中处理区扩散到相邻的香脂冷杉锯角叶蜂种群中的情况。老人塘试验区（2093公顷）的目的是确定NeabNPV能否扩散到喷雾带之间200米区域内的香脂冷杉锯角叶蜂种群中。NeabNPV通过一架配备了MicronAir AU4000旋转雾化器的Cessna 188B型农用飞机施用于处理区，施用量相当于每公顷1×10⁸个NeabNPV包涵体，溶于2.5升20%的糖水溶液中。在岛塘，喷雾后NeabNPV感染率随时间增加，尤其是靠近处理区的个体，感染率在距处理区400米的测量距离处下降。在老人塘，喷雾带之间区域的NeabNPV感染率上升得更高（80%对15%），锯角叶蜂密度下降得更多（84%对60%），高于对照区。
