Dworniczak Szymon, Niepsuj Grzegorz, Konofalski Leszek, Wojtyczka Robert D, Idzik Danuta, Kepa Maria, Wydmuch Zenobia, Pacha Jerzy, Mazur Wanda, Kieda-Szurkowska Jolanta, Nieroda-Müller Aleksandra, Kozielski Jerzy
Klinika Chorób Płuc i Gruźlicy SAM, Zabrze.
Pneumonol Alergol Pol. 2005;73(1):4-11.
The aim of the study was to perform microbiological and molecular analysis of Mycobacterium tuberculosis complex strains isolated from patients with pulmonary tuberculosis originating from two different regions of Silesia voivodship in 2000 year. One of 135 strains subjected to the study lacked IS6110 insertion sequence. Its belonging to Mycobacterium tuberculosis complex was confirmed by positive test for specific gyrB gene sequence. Strain resistance analysis revealed that in previously diagnosed patients resistance to streptomycin (16,2%) was more frequent than other. In contrast to never treated patients group where the prevalence of resistance to isoniazid was the most frequent (4,5%). In both group of patients resistance to rifampin or ethambutol was not detected. The multidrug resistance strains (4,6%) were detected only in the previously diagnosed group. Combined analysis of microbiological, epidemiological and molecular data are essential for creation of functional registry of patients infected with resistant Mycobacteria, giving the possibility of surveillance of drug resistant tuberculosis in populations.
该研究的目的是对2000年从西里西亚省两个不同地区的肺结核患者中分离出的结核分枝杆菌复合群菌株进行微生物学和分子分析。在接受研究的135株菌株中，有1株缺乏IS6110插入序列。通过对特定gyrB基因序列的阳性检测，证实其属于结核分枝杆菌复合群。菌株耐药性分析显示，在先前诊断的患者中，对链霉素的耐药性（16.2%）比其他情况更常见。与从未接受过治疗的患者组相比，在该组中对异烟肼的耐药率最高（4.5%）。在两组患者中均未检测到对利福平或乙胺丁醇的耐药性。多重耐药菌株（4.6%）仅在先前诊断的组中检测到。微生物学、流行病学和分子数据的综合分析对于建立感染耐药分枝杆菌患者的功能登记册至关重要，这使得对人群中耐药结核病进行监测成为可能。
