Baldwin Ann L, Primeau Richard L, Johnson William E
Department of Physiology, University of Arizona and Veterans Administration Medical Center, Tucson, AZ, USA.
J Am Assoc Lab Anim Sci. 2006 Jan;45(1):74-82.
To determine whether noise-induced stress disrupts the intestinal mucosa of laboratory rats, one group of 8 rats ("noise" rats) was subjected to 15 min of white noise (90 dB) daily for 3 wk. Another group ("quiet"rats) was housed for 3 wk in an acoustically similar room but with no additional noise. A 3rd group ("recovery" rats) was housed in the noise room for 3 wk and then in the quiet room for a further 3 wk. The ilea were fixed for microscopy. Villi adjacent to Peyer patches showed significantly more degranulated mast cells (mean+/-standard error of the mean, 3.95+/-0.80 versus 0.35+/-0.29, respectively) and eosinophils (mean+/-standard error of the mean, 9.46+/-0.44 versus 4.58+/-0.38) per villus section in noise rats than in quiet rats. Similar results were obtained with rooms reversed, to account for any differences in room characteristics. The mean width of villus laminar propria was significantly greater in noise rats than quiet rats, suggesting edema. In addition, mucosal epithelial cells of noise rats were often separated, sometimes detaching from the basement membrane, whereas those of quiet rats were intact. Behaviorally, noise rats exhibited significantly more grooming and rearing than quiet rats. Compared with noise rats, recovery rats showed no reduction in mast cell degranulation or mean width of villus lamina propria, but there were increased numbers of secreting goblet cells in villi adjacent to Peyer patches and some recovery of epithelial integrity.
为了确定噪声诱发的应激是否会破坏实验大鼠的肠黏膜，将一组8只大鼠（“噪声”组大鼠）每天置于90分贝的白噪声环境中15分钟，持续3周。另一组（“安静”组大鼠）在声学环境相似但无额外噪声的房间中饲养3周。第三组（“恢复”组大鼠）在噪声房间中饲养3周，然后在安静房间中再饲养3周。取出回肠进行显微镜检查。与派尔集合淋巴结相邻的绒毛中，“噪声”组大鼠每绒毛切片的脱颗粒肥大细胞（平均值±平均标准误分别为3.95±0.80和0.35±0.29）和嗜酸性粒细胞（平均值±平均标准误分别为9.46±0.44和4.58±0.38）明显多于“安静”组大鼠。将房间情况对调后也得到了类似结果，以排除房间特性差异的影响。“噪声”组大鼠绒毛固有层的平均宽度明显大于“安静”组大鼠，提示存在水肿。此外，“噪声”组大鼠的黏膜上皮细胞常常分离，有时会从基底膜脱落，而“安静”组大鼠的上皮细胞完整。行为学方面，“噪声”组大鼠表现出比“安静”组大鼠明显更多的梳理毛发和竖毛行为。与“噪声”组大鼠相比，“恢复”组大鼠的肥大细胞脱颗粒或绒毛固有层平均宽度没有降低，但派尔集合淋巴结相邻绒毛中分泌型杯状细胞数量增加，上皮完整性也有所恢复。
