Kordower J H, Bartus R T, Bothwell M, Schatteman G, Gash D M
Department of Neurobiology and Anatomy, University of Rochester School of Medicine 14642.
J Comp Neurol. 1988 Nov 22;277(4):465-86. doi: 10.1002/cne.902770402.
A monoclonal antibody raised against the receptor for nerve growth factor (NGF) was used to examine the distribution and morphology of NGF receptor-containing neurons within the central nervous system of Cebus apella monkeys. Most somata demonstrating positive immunoreactivity were localized within the Ch1-4 regions of the basal forebrain. Neurons in the Ch1 region displayed morphological features typical of cholinergic medial septal neurons. These perikarya were primarily vertically oriented (40-50 micron along the vertical axis) with both apical and basal neuritic processes. Magnocellular (40-50 micron) neurons within the Ch2 (vertical limb of the diagonal band), Ch3 (horizontal limb of the diagonal band) and Ch4 (nucleus basalis of Meynert) regions were multipolar and had rounded perikarya that often displayed an eccentric nucleus. Fibers presumably originating from the Ch1-2 regions were observed throughout the fimbria-fornix system and were found to terminate preferentially within the CA1 and CA3 regions of the hippocampal formation and within the dentate gyrus of the hippocampus. An intense fiber network was also observed in the olfactory tubercle and other rhinencephalic structures, presumably originating from the Ch3 region of the basal forebrain. Beaded processes emanating from the Ch4 region primarily coursed within the external capsule and terminated preferentially within layers I, II, and IV of the cerebral cortex. In a pattern similar to that of cortical acetylcholinesterase (AChE) staining, NGF receptor immunopositive fibers were oriented in a tangential plane within the molecular layer of the cortex and in both a radial and tangential fashion within the cortical granular cell layers. In addition to neural innervation, there was an extensive vascular apposition by NGF receptor-containing neurites on both large caliber vessels and microcapillaries. NGF receptor immunoreactivity was extensively, but not exclusively, colocalized with choline acetyltransferase (ChAT) and AChE in the basal forebrain. A small population of cholinergic neurons were observed that were not NGF receptor-immunoreactive. Conversely, a few NGF receptor-containing neurons that were noncholinergic were also observed in this brain region. NGF receptor-containing somata were also identified in the putamen. The number of immunoreactive neurons observed in this structure, however, would not appear to be sufficient to account for the homologous NGF receptor binding densities described in rodents.(ABSTRACT TRUNCATED AT 250 WORDS)
一种针对神经生长因子（NGF）受体产生的单克隆抗体，被用于检测僧帽猴中枢神经系统内含有NGF受体的神经元的分布和形态。大多数显示阳性免疫反应的胞体位于基底前脑的Ch1-4区域。Ch1区域的神经元呈现出胆碱能内侧隔核神经元的典型形态特征。这些胞体主要垂直排列（垂直轴上40-50微米），有顶端和基底神经突。Ch2（斜角带垂直支）、Ch3（斜角带水平支）和Ch4（迈内特基底核）区域的大细胞（40-50微米）神经元是多极的，有圆形胞体，其细胞核常偏位。推测起源于Ch1-2区域的纤维在整个穹窿-海马伞系统中都能观察到，并且优先终止于海马结构的CA1和CA3区域以及海马齿状回内。在嗅结节和其他嗅脑结构中也观察到一个密集的纤维网络，推测起源于基底前脑的Ch3区域。从Ch4区域发出的串珠状突起主要在外部囊内走行，并优先终止于大脑皮层的I、II和IV层。与皮质乙酰胆碱酯酶（AChE）染色模式相似，NGF受体免疫阳性纤维在皮层分子层内呈切线方向排列，在皮质颗粒细胞层内呈放射状和切线状排列。除了神经支配外，含有NGF受体的神经突在大口径血管和微毛细血管上有广泛的血管附着。在基底前脑，NGF受体免疫反应性广泛但并非完全与胆碱乙酰转移酶（ChAT）和AChE共定位。观察到一小部分胆碱能神经元没有NGF受体免疫反应性。相反，在这个脑区也观察到一些不含胆碱能的NGF受体阳性神经元。在壳核中也鉴定出了含有NGF受体的胞体。然而，在这个结构中观察到的免疫反应性神经元数量似乎不足以解释在啮齿动物中描述的同源NGF受体结合密度。（摘要截短至250字）
