Phillips Carrie L, Gattone Vincent H, Bonsib Stephen M
Division of Nephrology, Department of Medicine, Center for Biological Microscopy, Indiana University School of Medicine, Indianapolis, IN, USA.
Nephron Physiol. 2006;103(2):p75-81. doi: 10.1159/000090623. Epub 2006 Mar 10.
Glomerular capillary loops are complex vascular filters composed of interdigitating podocytes and fenestrated endothelial cells with an intervening proteoglycan-rich extracellular matrix. This arrangement is crucial to maintaining the filtration barrier but renders the glomerulus difficult to analyze by conventional two-dimensional histochemical techniques. When pathologic lesions distort glomerular architecture, its complex morphology is even more challenging to interpret. Fortunately, recent advances in microscopes and computer software now enable glomerular enthusiasts to dissect this complex structure with finer detail. In this review we explore the application of new methodologies such as two-photon microscopy that optimize three-dimensional, multicolor imaging and single-cell segmentation of glomerular components.
肾小球毛细血管袢是由相互交错的足细胞和有窗孔的内皮细胞以及其间富含蛋白聚糖的细胞外基质组成的复杂血管过滤器。这种结构对于维持滤过屏障至关重要，但使得肾小球难以通过传统的二维组织化学技术进行分析。当病理病变使肾小球结构变形时，其复杂的形态更具解释挑战性。幸运的是，显微镜和计算机软件的最新进展现在使肾小球研究人员能够更精细地剖析这种复杂结构。在本综述中，我们探讨了诸如双光子显微镜等新方法的应用，这些方法优化了肾小球成分的三维、多色成像和单细胞分割。
