Wade Christopher H, Umbarger Mark A, McAlear Michael A
Molecular Biology and Biochemistry Department, Wesleyan University, Middletown, CT 06459, USA.
Yeast. 2006 Mar;23(4):293-306. doi: 10.1002/yea.1353.
The ribosome biogenesis pathway constitutes one of the major metabolic obligations for a dividing yeast cell and it depends upon the activity of hundreds of gene products to produce the necessary rRNA and ribosomal protein components. Previously, we reported that a set of 65 S. cerevisiae genes that function in the rRNA biosynthesis pathway are transcriptionally co-regulated as cells pass through a variety of physiological transitions. By analysing multiple microarray-based transcriptional datasets, we have extended that study and now suggest that the ribosomal and rRNA biosynthesis regulon contains over 200 genes. This regulon is distinct from the set of ribosomal protein genes, and the promoters of the expanded RRB gene set are highly enriched for the PAC and RRPE motifs. Since a similar pattern of organization and gene regulation can be recognized in C. albicans, the RRB regulon appears to be a conserved, extensive, and metabolically important group of genes.
核糖体生物合成途径是分裂酵母细胞的主要代谢任务之一，它依赖数百种基因产物的活性来产生必需的rRNA和核糖体蛋白组分。此前，我们报道过，随着细胞经历各种生理转变，一组在rRNA生物合成途径中起作用的65个酿酒酵母基因会受到转录共调控。通过分析多个基于微阵列的转录数据集，我们扩展了该项研究，现在认为核糖体和rRNA生物合成调节子包含200多个基因。这个调节子与核糖体蛋白基因集不同，并且扩展后的RRB基因集的启动子高度富集PAC和RRPE基序。由于在白色念珠菌中可以识别出类似的组织和基因调控模式，RRB调节子似乎是一组保守、广泛且在代谢上很重要的基因。
