Zhao Shulin, Wang Bing, Yuan Hongyan, Xiao Dan
College of Chemical Engineering, Sichuan University, Chengdu 610065, China.
J Chromatogr A. 2006 Aug 4;1123(1):138-41. doi: 10.1016/j.chroma.2006.05.038. Epub 2006 Jul 3.
A capillary electrophoresis (CE)/optical fiber light-emitting diode (LED)-induced fluorescence detection method is developed for the determination of agmatine in biological samples. The agmatine was precolumn-derivatized with fluorescence tagging reagent, fluorescein isothiocyanate (FITC). Optimal separation and determination for agmatine were obtained with an electrophoretic buffer of 20 mM sodium borate (pH 9.2). Under the optimal conditions, the determination of agmatine was achieved in less than 4 min, and the detection limit was 4.1x10(-9) M (S/N = 3). The relative standard deviation (RSD) for 11 parallel determination of agmatine was less than 3.0%. The present CE-LED induced fluorescence detection method has been applied to detect agmatine in rat brain tissue, rat stomach tissue, human serum, and human urine. The level of agmatine in human urine was quantified by CE for the first time and found to be in the range 2.5-4.1x10(-7) M.
建立了一种毛细管电泳（CE）/光纤发光二极管（LED）诱导荧光检测法，用于测定生物样品中的胍丁胺。胍丁胺用荧光标记试剂异硫氰酸荧光素（FITC）进行柱前衍生。使用20 mM硼酸钠（pH 9.2）的电泳缓冲液可实现胍丁胺的最佳分离和测定。在最佳条件下，不到4分钟即可完成胍丁胺的测定，检测限为4.1×10（-9）M（S/N = 3）。胍丁胺11次平行测定的相对标准偏差（RSD）小于3.0%。目前的CE-LED诱导荧光检测法已应用于大鼠脑组织、大鼠胃组织、人血清和人尿液中胍丁胺的检测。首次通过CE对人尿液中胍丁胺的含量进行了定量，发现其范围为2.5-4.1×10（-7）M。
