Zheng Xianrui, Ning Chao, Dong Yichun, Zhao Pengju, Li Junhui, Fan Ziyao, Li Jiang, Yu Ying, Mrode Raphael, Liu Jian-Feng
National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Agricultural Commission of Haian County, Jiangsu Province, Haian 226600, China.
Biochem Biophys Res Commun. 2017 Dec 9;494(1-2):292-297. doi: 10.1016/j.bbrc.2017.10.038. Epub 2017 Oct 9.
Mammary gland is an important organ for milk synthesis and secretion. It undergoes dramatic physiological changes to adapt the shift from peak to late lactation stage. Protein plays a final very vital role in many life functions, and the protein changes during different lactation stages potentially reflect the biology of lactation and the functions of mammary gland in cows. In current study, we adopted tandem mass tags label-based quantitative analysis technique and to investigate proteome changes occurring in bovine mammary gland from peak to late lactation stages. A total of 3753 proteins from mammary tissues taken at two lactation points from four individual cows by biopsy were quantified, out of which 179 proteins were expressed differentially between two stages. We observed five new DEPs (AACS, DHCR7, GSTM3, SFRP1 and SFRP4) and nine functional well-studies known proteins (PLIN2, LPIN1, PLIN3, GSN, CD74, MMP2, SOD1, SOD3 and GPX3) related to milk performance and mammary morphology. Bioinformatics analyses of the DEPs showed a majority of the up-regulated proteins during late lactation stage were related to apoptosis and immune process, while the downregulated proteins were mainly involved in localization, lipid metabolic and transport process. This suggests that the mammary gland can adapt to different molecular functions according to the biological need of the animal. From the integrated analysis of the differentially expressed proteins with known quantitative trait loci and genome-wide association study data, we identified 95 proteins may potentially affect milking performance. We expect findings in this study could be a valuable resource for future studies investigating the bovine proteome and functional studies.
乳腺是乳汁合成和分泌的重要器官。它经历显著的生理变化以适应从泌乳高峰期到后期的转变。蛋白质在许多生命功能中起着至关重要的最终作用，奶牛不同泌乳阶段的蛋白质变化可能反映泌乳生物学以及乳腺的功能。在本研究中，我们采用基于串联质量标签标记的定量分析技术，研究奶牛乳腺从泌乳高峰期到后期阶段发生的蛋白质组变化。通过活检从四头个体奶牛的两个泌乳点采集的乳腺组织中共定量了3753种蛋白质，其中179种蛋白质在两个阶段之间差异表达。我们观察到五个新的差异表达蛋白（AACS、DHCR7、GSTM3、SFRP1和SFRP4）以及九个功能研究充分的已知蛋白（PLIN2、LPIN1、PLIN3、GSN、CD74、MMP2、SOD1、SOD3和GPX3）与产奶性能和乳腺形态有关。对差异表达蛋白的生物信息学分析表明，泌乳后期阶段大多数上调的蛋白质与细胞凋亡和免疫过程有关，而下调的蛋白质主要参与定位、脂质代谢和运输过程。这表明乳腺可以根据动物的生物学需求适应不同的分子功能。通过对差异表达蛋白与已知数量性状位点和全基因组关联研究数据的综合分析，我们确定了95种可能潜在影响挤奶性能的蛋白质。我们期望本研究的结果能为未来研究牛蛋白质组和功能研究提供有价值的资源。
