Klejdus Borivoj, Moravcová Jitka, Lojková Lea, Vacek Jan, Kubán Vlastimil
Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry Brno, Czech Republic.
J Sep Sci. 2006 Feb;29(3):378-84. doi: 10.1002/jssc.200500421.
A method for the simultaneous determination of 4(5)-methylimidazole (4MeI) and 2-acetyl-4(5)-(1,2,3,4-tetrahydroxybutyl)-imidazole (THI) was developed using SPE and HPLC/MS. Solid-phase extraction using SCX Disc cartridges was used for isolation of the analytes from liquid samples. The lower LOQwas 0.1 ng/mL for 4MeI and 0.2 ng/ mL for THI. The linearity of the calibration curves was satisfactory as indicated by correlation coefficients >0.999. The CV for the intra- and inter-day precision was <5% (n = 6); the accuracy was in the range 98-103%. The recovery was > or = 97 and > or = 98% for THI and 4MeI, respectively. The method was used to determine THI and 4MeI in beverages, coffee, caramel colours and other samples.
建立了一种使用固相萃取（SPE）和高效液相色谱/质谱（HPLC/MS）同时测定4（5）-甲基咪唑（4MeI）和2-乙酰基-4（5）-（1,2,3,4-四羟基丁基）-咪唑（THI）的方法。使用强阳离子交换（SCX）盘式固相萃取柱从液体样品中分离分析物。4MeI的最低定量限（LOQ）为0.1 ng/mL，THI为0.2 ng/mL。校准曲线的线性令人满意，相关系数>0.999。日内和日间精密度的变异系数（CV）<5%（n = 6）；准确度在98-103%范围内。THI和4MeI的回收率分别≥97%和≥98%。该方法用于测定饮料、咖啡、焦糖色素和其他样品中的THI和4MeI。
