Kharrazi H, Vaisi Raygani A, Sabokroh A R, Pourmotabbed T
Department of Medical Biochemistry, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Clin Biochem. 2006 Jun;39(6):613-6. doi: 10.1016/j.clinbiochem.2006.02.002. Epub 2006 Mar 20.
Coronary artery disease (CAD) is the major cause of death in developing countries, such as Iran. The apolipoprotein E gene (APOE) is considered an important genetic determinant of CAD. In this study, the relationship between APOE polymorphism with lipid variation in CAD patients in Kermanshah, Iran was investigated.
This case-control study consisted of 115 CAD patients who angiographically had at least 30% stenosis and 135 unrelated controls. APOE polymorphism was detected by PCR-RFLP and serum lipid level was measured enzymatically.
The APOE-epsilon4 and -epsilon2 allele frequencies were significantly higher in the CAD patients than in the control group (P < 0.001). The CAD patients with epsilon3/epsilon4 genotype had also higher TC (P < 0.001) and LDL-C (P < 0.01) and lower HDL-C (P < 0.03) levels than that of the control group.
APOE-epsilon4 allele is a risk factor for CAD, so that carriers of this allele with high levels of LDL-C may be susceptible to CAD and myocardial infarction.
冠状动脉疾病（CAD）是伊朗等发展中国家的主要死因。载脂蛋白E基因（APOE）被认为是CAD的一个重要遗传决定因素。在本研究中，调查了伊朗克尔曼沙赫CAD患者中APOE多态性与血脂变化之间的关系。
本病例对照研究包括115例经血管造影显示至少有30%狭窄的CAD患者和135例无关对照。通过PCR-RFLP检测APOE多态性，并用酶法测量血清脂质水平。
CAD患者中APOE-ε4和-ε2等位基因频率显著高于对照组（P<0.001）。与对照组相比，ε3/ε4基因型的CAD患者TC（P<0.001）和LDL-C（P<0.01）水平更高，HDL-C（P<0.03）水平更低。
APOE-ε4等位基因是CAD的一个危险因素，因此该等位基因携带者且LDL-C水平高可能易患CAD和心肌梗死。
