Ryan Daniel, Leukes Winton, Burton Stephanie
Department of Chemical Engineering, The University of Sydney, NSW 2006 Australia.
Bioresour Technol. 2007 Feb;98(3):579-87. doi: 10.1016/j.biortech.2006.02.001. Epub 2006 Mar 20.
The successful bioremediation of a phenolic wastewater by Trametes versicolor was found to be dependent on a range of factors including: fungal growth, culture age and activity and enzyme (laccase) production. These aspects were enhanced by the optimisation of the growth medium used and time of addition of the pollutant to the fungal cultures. Different media containing 'high' (20 g/L), 'low' (2 g/L) and 'sufficient' (10 g/L) concentrations of carbon and nitrogen sources were investigated. The medium containing both glucose and peptone at 10 g/L resulted in the highest Growth Related Productivity (the product of specific yield and micro) of laccase (1.46 Units of laccase activity)/gram biomass/day and was used in all further experiments. The use of the guaiacol as an inducer further increased laccase activity 780% without inhibiting growth; similarly the phenolic effluent studied boosted activity almost 5 times. The timing of the addition of the phenolic effluent was found to have important consequences in its removal and at least 8 days of prior growth was required. Under these conditions, 0.125 g phenol/g biomass and 0.231 g o-cresol/g biomass were removed from solution per day.
研究发现，云芝对含酚废水的成功生物修复取决于一系列因素，包括：真菌生长、培养时间和活性以及酶（漆酶）的产生。通过优化所用生长培养基和向真菌培养物中添加污染物的时间，这些方面得到了增强。研究了含有“高”（20 g/L）、“低”（2 g/L）和“充足”（10 g/L）碳氮源浓度的不同培养基。含有10 g/L葡萄糖和蛋白胨的培养基产生了最高的与生长相关的漆酶生产率（比产率和微生物量的乘积）（1.46漆酶活性单位/克生物量/天），并用于所有进一步的实验。使用愈创木酚作为诱导剂可进一步提高漆酶活性780%，且不抑制生长；同样，所研究的含酚废水可使活性提高近5倍。发现添加含酚废水的时间对其去除有重要影响，至少需要提前8天生长。在这些条件下，每天从溶液中去除0.125 g苯酚/克生物量和0.231 g邻甲酚/克生物量。
