Ryan Daniel R, Leukes Winston D, Burton Stephanie G
Department of Chemical Engineering, The University of Sydney, Sydney, Australia.
Biotechnol Prog. 2005 Jul-Aug;21(4):1068-74. doi: 10.1021/bp049558r.
Of the various types of industry-generated effluents, those containing organic pollutants such as phenols are generally difficult to remediate. There is a need to develop new technologies that emphasize the destruction of these pollutants rather than their disposal. In this work the white rot fungus, Trametes pubescens, was demonstrated to be an effective bioremediation agent for the treatment of phenolic wastewaters. An airlift loop reactor was optimized, in terms of volumetric oxygen transfer rate (K(L)a = 0.45 s(-1)), to provide an environment suited to rapid growth of T.pubescens (mu = 0.25 day(-1)) and a particularly efficient growth yield on glucose of 0.87 g biomass.g glucose(-1). The phenolic effluent was shown to be a paramorphogen, influencing fungal pellet morphology in the reactor, as well as increasing laccase enzyme activity by a factor of 5 over the control, to a maximum of 11.8 U.mL(-1). This increased activity was aided by the feeding of nonrepressing amounts (0.5 g.L(-1)) of glucose to the reactor culture. To our knowledge the degradation results represent the highest rate of removal (0.033 g phenol.g biomass(-1).day(-1)) of phenolic compounds from water reported for white rot fungi.
在各类工业产生的废水中，那些含有酚类等有机污染物的废水通常难以修复。需要开发强调破坏这些污染物而非将其处置的新技术。在这项工作中，白腐真菌毛栓菌被证明是处理含酚废水的一种有效生物修复剂。对气升式环流反应器进行了优化，使其体积氧传递速率（K(L)a = 0.45 s(-1)）达到适宜毛栓菌快速生长（μ = 0.25 天(-1)）的环境，且其在葡萄糖上的生长产率特别高效，达到 0.87 g 生物量·g 葡萄糖(-1)。含酚废水被证明是一种形态发生原，影响反应器中真菌菌球的形态，并且相较于对照，漆酶活性提高了 5 倍，最高达到 11.8 U·mL(-1)。向反应器培养物中添加非抑制量（0.5 g·L(-1)）的葡萄糖有助于提高这种活性。据我们所知，这些降解结果代表了白腐真菌从水中去除酚类化合物的最高速率（0.033 g 苯酚·g 生物量(-1)·天(-1)）。
