Facultad de Zootecnia, Universidad Autónoma de Chihuahua, Chihuahua, Chihuahua 31120, Mexico.
Molecules. 2009 Oct 16;14(10):4159-65. doi: 10.3390/molecules14104159.
This study examined the feasibility of using Trametes sp. grown under drought conditions to catalyze the covalent cross-linking of ferulated arabinoxylans. The rate of polymerization of ferulated arabinoxylans solutions at 0.5% (w/v) was dose dependent on the laccase concentration in the system. Above 1.2 nkat laccase/mg ferulated arabinoxylan, the rate of cross-linking reached a plateau, suggesting that the reaction reached saturation. For 2% (w/v) ferulated arabinoxylans treated with laccase (1.6 nkat/mg ferulated arabinoxylan), stable gels were formed after 4 h at 25 degrees C, with cross-linking contents (diferulic and triferulic acid) contents of 0.03 and 0.015 microg/mg ferulated arabinoxylan, respectively. This study demonstrated that Trametes sp. can be a source of biopolymer cross-linking enzymes like laccase.
本研究考察了在干旱条件下生长的云芝（Trametes sp.）用于催化阿魏酰阿拉伯木聚糖共价交联的可行性。在 0.5%（w/v）浓度下，阿魏酰阿拉伯木聚糖溶液的聚合速率与体系中的漆酶浓度呈剂量依赖性。在 1.2 nkat 漆酶/mg 阿魏酰阿拉伯木聚糖以上，交联速率达到平台，表明反应达到饱和。对于用漆酶（1.6 nkat/mg 阿魏酰阿拉伯木聚糖）处理的 2%（w/v）阿魏酰阿拉伯木聚糖，在 25 摄氏度下 4 小时后形成稳定的凝胶，交联含量（二糠基和三糠基酸）分别为 0.03 和 0.015 microg/mg 阿魏酰阿拉伯木聚糖。本研究表明，云芝（Trametes sp.）可以成为像漆酶这样的生物聚合物交联酶的来源。
