Nuccitelli Richard, Pliquett Uwe, Chen Xinhua, Ford Wentia, James Swanson R, Beebe Stephen J, Kolb Juergen F, Schoenbach Karl H
Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, USA.
Biochem Biophys Res Commun. 2006 May 5;343(2):351-60. doi: 10.1016/j.bbrc.2006.02.181. Epub 2006 Mar 10.
We have discovered a new, drug-free therapy for treating solid skin tumors. Pulsed electric fields greater than 20 kV/cm with rise times of 30 ns and durations of 300 ns penetrate into the interior of tumor cells and cause tumor cell nuclei to rapidly shrink and tumor blood flow to stop. Melanomas shrink by 90% within two weeks following a cumulative field exposure time of 120 micros. A second treatment at this time can result in complete remission. This new technique provides a highly localized targeting of tumor cells with only minor effects on overlying skin. Each pulse deposits 0.2 J and 100 pulses increase the temperature of the treated region by only 3 degrees C, ten degrees lower than the minimum temperature for hyperthermia effects.
我们发现了一种治疗实体皮肤肿瘤的全新无药疗法。强度大于20 kV/cm、上升时间为30纳秒且持续时间为300纳秒的脉冲电场能够穿透肿瘤细胞内部，使肿瘤细胞核迅速收缩，并导致肿瘤血流停止。黑色素瘤在累计电场暴露时间达到120微秒后的两周内缩小90%。此时进行第二次治疗可实现完全缓解。这项新技术能够高度局部靶向肿瘤细胞，对覆盖其上的皮肤仅有轻微影响。每个脉冲释放0.2焦耳能量，100个脉冲仅使治疗区域温度升高3摄氏度，比产生热疗效果的最低温度低10摄氏度。
