Yano Isao, Hoshino Rie
Department of Marine Bioscience, Fukui Prefectural University, Obama, Fukui 917-0003, Japan.
Comp Biochem Physiol A Mol Integr Physiol. 2006 May;144(1):18-23. doi: 10.1016/j.cbpa.2006.01.026. Epub 2006 Mar 20.
The effects of 17 beta-estradiol on induction of vitellogenin synthesis and oocyte development were investigated in previtellogenic ovary of immature kuruma prawn (Marsupenaeus japonicus) incubated with Medium 199. After three days incubation of previtellogenic ovary, Vg concentrations in media containing 3.6 nM, 36.7 nM, 367 nm and 3671 nM 17 beta-estradiol were significantly (p<0.01) greater than that of Ringer solution or the pure ethanol vehicle. Furthermore, a more advanced stage of oocyte development at oil globule stage (primary vitellogenic stage), which is surrounded by round and greatly expanded follicle cells, was observed in previtellogenic ovarian pieces incubated in media containing 3.6, 36.7, 367 and 3671 nM 17 beta-estradiol. The results of these studies show that 17 beta-estradiol induces Vg synthesis and appearance of primary vitellogenic oocyte in the ovary of immature prawns.
在含有199培养基的未成熟日本对虾（Marsupenaeus japonicus）的卵黄发生前卵巢中，研究了17β-雌二醇对卵黄蛋白原合成诱导和卵母细胞发育的影响。卵黄发生前卵巢孵育三天后，含有3.6 nM、36.7 nM、367 nM和3671 nM 17β-雌二醇的培养基中的卵黄蛋白原浓度显著（p<0.01）高于林格氏液或纯乙醇载体。此外，在含有3.6、36.7、367和3671 nM 17β-雌二醇的培养基中孵育的卵黄发生前卵巢切片中，观察到处于油球阶段（初级卵黄发生阶段）的卵母细胞发育到了更高级阶段，该阶段被圆形且大幅扩张的卵泡细胞包围。这些研究结果表明，17β-雌二醇可诱导未成熟对虾卵巢中卵黄蛋白原的合成以及初级卵黄发生卵母细胞的出现。
