Tishkov Vladimir I, Popov Vladimir O
Department of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow 119992, Russia.
Biomol Eng. 2006 Jun;23(2-3):89-110. doi: 10.1016/j.bioeng.2006.02.003. Epub 2006 Mar 20.
NAD+-dependent formate dehydrogenase (FDH, EC 1.2.1.2) is one of the best enzymes for the purpose of NADH regeneration in dehydrogenase-based synthesis of optically active compounds. Low operational stability and high production cost of native FDHs limit their application in commercial production of chiral compounds. The review summarizes the results on engineering of bacterial and yeast FDHs aimed at improving their chemical and thermal stability, catalytic activity, switch in coenzyme specificity from NAD+ to NADP+ and overexpression in Escherichia coli cells.
烟酰胺腺嘌呤二核苷酸（NAD⁺）依赖性甲酸脱氢酶（FDH，EC 1.2.1.2）是基于脱氢酶的光学活性化合物合成中用于烟酰胺腺嘌呤二核苷酸（NADH）再生的最佳酶之一。天然FDH的操作稳定性低和生产成本高限制了它们在手性化合物商业生产中的应用。本综述总结了针对细菌和酵母FDH进行工程改造的结果，旨在提高其化学和热稳定性、催化活性、辅酶特异性从NAD⁺转换为烟酰胺腺嘌呤二核苷酸磷酸（NADP⁺）以及在大肠杆菌细胞中的过表达。
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